We narrowed to 7,815 results for: Lif

1. pMRX-No-HaloTag7-mGFP-LC3

   Plasmid

   #184901

   Purpose

   Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   LC3B
   

   Use

   Tags

   HaloTag7 and monomeric EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCLBW cox8 EGFP mCherry

   Plasmid

   #78520

   Purpose

   Fluorescent mitophagy reporter

   Depositor

   David Chan

   Article

   Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

   Insert

   none
   

   Use

   Retroviral

   Tags

   cox8 EGFP mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTK645

   Plasmid

   #114711

   Purpose

   Express NuMA(1700-2115)-RFP-Nano from Rosa 26 locus

   Depositor

   Tomomi Kiyomitsu

   Article

   Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

   Insert

   NuMA(1700-2115)-RFP-Nano
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKanCMV_mRuby3

   Plasmid

   #172854

   Purpose

   mRuby3 expressed under the CMV promotor

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   mRuby3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR OVA/p2a/mCherry-CaaX

   Plasmid

   #113030

   Purpose

   for lentivirus production of ovalbumin and mcherry-caax

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   OVA/p2a/mCherry-CaaX
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTol2-elavl3_NES-rsCaMPARI-mRuby3

   Plasmid

   #122129

   Purpose

   Pan-neuronal expression of rsCaMPARI in zebrafish, nucleus excluded

   Depositor

   Eric Schreiter

   Article

   Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

   Insert

   rsCaMPARI
   

   Use

   Zebrafish expression

   Tags

   NES-His and mRuby3

   Expression

   Mutation

   Promoter

   elavl3

   Available since

   Oct. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. nSYB-GAL80-DD

   Plasmid

   #124778

   Purpose

   Expresses GAL80 fused with destabilizing domain (DD) downstream of a n-Synaptobrevin promoter

   Depositor

   Jing-W Wang

   Article

   Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

   Insert

   TMP-inducible destabilized GAL80 (GAL80-DD)
   

   Use

   Tags

   DD

   Expression

   Insect

   Mutation

   Promoter

   nSynaptobrevin

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-jAspSnFR3-mRuby3

   Plasmid

   #203498

   Purpose

   jAspSnFR3-mRuby3 in lentiviral expression vector

   Depositor

   Lucas Sullivan

   Article

   Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

   Insert

   jAspSnFR3-mRuby3
   

   Use

   Lentiviral

   Tags

   His-tag and mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-TRE-mTurquoise2-WPRE

   Plasmid

   #104110

   Purpose

   AAV-mediated expression of mTurquoise2 under the TRE promoter.

   Depositor

   Takeshi Imai

   Article

   Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

   Insert

   mTurquoise2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   TRE

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMK33/Cas9

    Plasmid

    #133781

    Purpose

    Expresses drosophila-optimized Cas9

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

    Insert

    Drosophila-optimized Cas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. rGluA2 (px330:2-guideCas9)

    Plasmid

    #169439

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEW8

    Plasmid

    #84238

    Purpose

    Plasmid containing a P2A peptide (porcine teschovirus-1 derived) sequence that works efficiently in Saccharomyces cerevisiae

    Depositor

    David Weinberg

    Article

    DiSanto et al Elife. 2016 Oct 1;5. pii: e20069. doi: 1

    Insert

    HA-mRuby-P2A-eGFP
    

    Use

    Tags

    HA-mRuby and eGFP

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2

    Plasmid

    #205161

    Purpose

    express β-ACTIN-3′UTR-24xMS2

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    β-ACTIN-3′UTR-24xMS2
    

    Use

    Tags

    tagBFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Syn1-tTA

    Plasmid

    #104109

    Purpose

    AAV-mediated expression of tTA under the Syn1 promoter.

    Depositor

    Takeshi Imai

    Article

    Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

    Insert

    tTA2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-TRE-tdTomato-WPRE

    Plasmid

    #104112

    Purpose

    AAV-mediated expression of tdTomato under the TRE promoter.

    Depositor

    Takeshi Imai

    Article

    Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-TRE-EYFP-WPRE

    Plasmid

    #104111

    Purpose

    AAV-mediated expression of EYFP under the TRE promoter.

    Depositor

    Takeshi Imai

    Article

    Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDestTol2-QUAS:GCaMP7a

    Plasmid

    #184817

    Purpose

    Used to generate the Tg(QUAS:GCaMP7a)c594 transgenic line. GCaMP7a from Muto et al., 2013 (https://doi.org/10.1016/j.cub.2012.12.040).

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    QUAS:GCaMP7a-pA
    

    Use

    Zebrafish tol2 transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUbC-tdTomato

    Plasmid

    #183710

    Purpose

    tdTomato expression from UbiC promoter

    Depositor

    Jeffrey Gerst

    Article

    Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

    Insert

    tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    UbiC

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEMHE-NLS-mEGFP

    Plasmid

    #105527

    Purpose

    T7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signal

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Nucleoplasmin
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    NLS of nucleoplasmin (encoding amino acids KRPAAT…

    Promoter

    T7

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKL11-GCaMP6f bb100

    Plasmid

    #158983

    Purpose

    Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    GCaMP6f
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#100833

    Promoter

    Bba_J23100 (sigma 70)

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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