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KTK_032

Plasmid

#180528

Purpose

Entry vector containing pLac promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

pLac promoter

Expression

Bacterial

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_045

Plasmid

#180543

Purpose

Entry vector containing RBS 30,000 a.u. designed strength

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sRBS 30,000 a.u. designed strength

Expression

Bacterial

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_034

Plasmid

#180526

Purpose

Entry vector containing pLux promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

pLux promoter

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_252

Plasmid

#180553

Purpose

Entry vector containing mScarlet in E1.3b format

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

mScarlet in E1.3b format

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_046

Plasmid

#180544

Purpose

Entry vector containing RBS 250 a.u. designed strength

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sRBS 250 a.u. designed strength

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_042

Plasmid

#180530

Purpose

Entry vector containing J23100 anderson promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

J23100 anderson promoter

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-037 NLSNoLS(D-G)//Esp3I

Plasmid

#130239

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

NLSNoLS

Use

Synthetic Biology

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-038 Lyn(D-G)//Esp3I

Plasmid

#130240

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

Lyn

Use

Synthetic Biology

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-039 LifeAct(D-G)//Esp3I

Plasmid

#130241

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

LifeAct

Use

Synthetic Biology

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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mApple-ERM

Plasmid

#217377

Purpose

ER marker

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

rat cb5 ERM

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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KTK_139

Plasmid

#180565

Purpose

Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_113

Plasmid

#180540

Purpose

Entry vector containing Signal peptide region of CsgA including SecB-N22 sequenced

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Signal peptide region of CsgA including SecB-N22 sequenced

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_095

Plasmid

#180524

Purpose

dCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assembly

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_324

Plasmid

#180566

Purpose

Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-021 Lamp1-Sirius//BsaI

Plasmid

#130202

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

Lamp1-Sirius

Use

Synthetic Biology

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-012 Vimentin-SuperNova//BsaI

Plasmid

#130193

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

Vimentin-SuperNova

Use

Synthetic Biology

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-029 Vimentin-mVenus//BsaI

Plasmid

#130210

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

Vimentin-mVenus

Use

Synthetic Biology

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-024 Connector(D-G)//Esp3I

Plasmid

#130226

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

artificial connector

Use

Synthetic Biology

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-025 eGFP(I-J)//Esp3I

Plasmid

#130227

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

eGFP

Use

Synthetic Biology

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-026 mCherry(I-J)//Esp3I

Plasmid

#130228

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

mCherry

Use

Synthetic Biology

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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