We narrowed to 15,989 results for: LEA

1. pDY0312 PhiBT Integrase

   Plasmid

   #179111

   Purpose

   PhiBT Integrase expression vector

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   PhiBT Integrase
   

   Use

   CRISPR

   Available Since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CRHR2-DuET

   Plasmid

   #213216

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CRHR2 (CRHR2 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TVBB C-term-mNeongreen-Blast

   Plasmid

   #169229

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Blast at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mNeongreen-P2A-Blast
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pclbw-opa1(isoform 7DeltaS1)-myc

   Plasmid

   #62846

   Purpose

   Mammalian Expression of myc-tagged Opa1 isoform 7 Delta S1

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   mmOPA1isoform7 DeltaS1 - myc (Opa1 Mouse)
   

   Use

   Retroviral

   Tags

   9xMyc

   Expression

   Mammalian

   Promoter

   cmv-bactin

   Available Since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nickase SpCas9 - pCMV-T7-nSpCas9(H840A)-P2A-GFP (HES140)

   Plasmid

   #208976

   Purpose

   Nickase SpCas9 (H840A) with a P2A GFP and 3xFLAG, expressed from a CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   nSpCas9(H840)-BPNLS-P2A-GFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-hM₃O-IP

   Plasmid

   #46645

   Depositor

   Nobuaki Kikyo

   Article

   Hirai et al Stem Cells. 2011 Sep;29(9):1349-61. doi:

   Insert

   hM₃O (POU5F1 Human)
   

   Use

   Retroviral

   Tags

   IRES Puro and human MyoD (aa 1-62)

   Expression

   Mammalian

   Mutation

   contains a fragment of human MyoD (aa1-62) fused …

   Available Since

   July 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-nEF Con/Foff hChR2(H134R)-EYFP

   Plasmid

   #55647

   Purpose

   Cre-on/Flp-off ChR2-EYFP under the short Ef1a promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV; Cre on/flp off chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   Short Ef1a

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 AIF-mClover

   Plasmid

   #129575

   Purpose

   Expression of mClover fused AIF

   Depositor

   Stephen Tait

   Article

   Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

   Insert

   AIF (AIFM1 Human)
   

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC13P-iCLHN

   Plasmid

   #66580

   Purpose

   CLYBL safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-C13 TALENs

   Depositor

   Jizhong Zou

   Article

   Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

   Insert

   Nanoluc-HaloTag
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-shMcu-2

    Plasmid

    #181867

    Purpose

    Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter

    Depositor

    Hilmar Bading

    Article

    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

    Insert

    mitochondrial calcium uniporter (Mcu Mouse)
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    hrGFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDB-His-MBP-mGSDMD

    Plasmid

    #123365

    Purpose

    Expression of full-length mouse GSDMD in E. coli

    Depositor

    Hao Wu

    Article

    Orning et al Science. 2018 Nov 30;362(6418):1064-1069

    Insert

    GSDMD (Gsdmd Mouse)
    

    Tags

    His6-MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKM-U6-reRNA-hsHEKsite_1_3

    Plasmid

    #205638

    Purpose

    reRNA guide targeting HEKsite_1_3

    Depositor

    Gerald Schwank

    Article

    Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

    Insert

    reRNA-HEKsite_1_3 (EPHA3 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLU-CMV-Flag-hATG5 WT

    Plasmid

    #111803

    Purpose

    Mammalian Expression of hATG5

    Depositor

    Jun Hee Lee

    Article

    Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

    Insert

    hATG5 (ATG5 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

    Plasmid

    #176534

    Purpose

    Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    CRISPR-associated IscB (Delaware Bay metagenome)
    

    Tags

    14xHis-MBP-bdSUMO and TwinStrep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LaminA-ECFP-SNAP

    Plasmid

    #187965

    Purpose

    expresses LaminA with C-terminal tags ECFP and SNAP

    Depositor

    Markus Wahl

    Article

    Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

    Insert

    LaminA (LMNA Human)
    

    Tags

    ECFP-SNAP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-BRD4ΔN-miRFP670-Cry2WT

    Plasmid

    #122439

    Purpose

    Expresses disordered protein BRD4(462-1362) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    Cry2WT and miRFP670

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-461

    Promoter

    SFFV

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCOLADuet-1_nsp10 (SARS-CoV-2)

    Plasmid

    #169158

    Purpose

    To express SARS-CoV-2 nsp10 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp10 (ORF1ab SARS-CoV-2)
    

    Tags

    Methionine

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV2-SBECN1W

    Plasmid

    #107937

    Purpose

    Adeno-Associated viral (AAV) vector plasmid expressing mouse Beclin-1 (BECN1) in neurons

    Depositor

    Johan Jakobsson

    Article

    Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

    Insert

    mouse Beclin-1 (BECN1) (Becn1 Mouse)
    

    Use

    AAV

    Promoter

    hSynapsin

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX MLKL(wt)--2A-mCherry-puro

    Plasmid

    #231974

    Purpose

    Tet inducible expression of DmrB-Mlkl with mCherry to induce WT Mlkl expression

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    MLKL WT construct with bi-cistronic mCherry (Mlkl Mouse)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP4-BG505.SOSIP-QES.i03.c01-8his

    Plasmid

    #111846

    Purpose

    Mammalian expression plasmid for soluble BG505 SOSIP.664; QES mutant with enhanced binding to PG16

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 Env (BG505 SOSIP.664)
    

    Tags

    8his purification tag and CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; has SOSIP mutatio…

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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