We narrowed to 7,660 results for: Pol
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Plasmid#210940PurposeInsect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.DepositorInsertDCAF12:M1-S453 (DCAF12 Human)
ExpressionInsectMutationR131Q natural variant (VAR_035322)PromoterPolyhedrinAvailable SinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_WDR19:1-636
Plasmid#210933PurposeInsect expression of human WDR19 fragment M1 to G636. N-terminal 6X His tag with TEV cleavage.DepositorInsertWDR19:M1-G636 (WDR19 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_LRRK2:2141-2527
Plasmid#210901PurposeInsect expression of human LRRK2 fragment T2141 to E2527. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertLRRK2:T2141-E2527 (LRRK2 Human)
TagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationM2397T natural variant ( VAR_024965)PromoterPolyhedrinAvailable SinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLX304-EZH1-D745N
Plasmid#203585PurposeExpresses V5-tagged mutant version of EZH1 partially resistant to JQ-EZ-05 in mammalian cells.DepositorInsertEZH1 (EZH1 Human)
UseLentiviralTagsV5-taggedExpressionMammalianMutationchanged Aspartic Acid 745 to Asparagine for parti…PromoterCMVAvailable SinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFR*-CUbo(K48R K63R)-GFP
Plasmid#212818PurposeConstitutive or doxycycline-inducible expression of EGFR*-CUbo(K48R K63R)-GFP in mammalian cellsDepositorAvailable SinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
PABPC1_544-636_pGEX6P1
Plasmid#209789PurposeBacterial expression of PABPC1 Mlle domainDepositorInsertMlle domain of PABPC1 (PABPC1 Human)
TagsGSTExpressionBacterialMutationaa 544-636 onlyPromotertacAvailable SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_WT RAD23B_aa 80-409 (delta UBL domain)
Plasmid#201545PurposeExpresses a mutant form of human RAD23B that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23BDepositorInsertUV excision repair protein RAD23 homolog B (RAD23B Human)
TagsFLAGExpressionMammalianMutationLacks N-terminal residues 1-79Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-iCre-pA]-lox-Nedd1-mOrange2-rCM1
Plasmid#196879PurposeNeuron-specific expression of Need1 fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.DepositorAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3UTR
Plasmid#202550PurposeReady for cloning and heterologous expression of genes in mammalian cells with protein export to cultivation mediaDepositorTypeEmpty backboneTagsATG initiation codon followed by an IGKV3 signal …ExpressionMammalianPromoterCMVAvailable SinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBVboostFGII WPRE 3C-CVB3
Plasmid#202077PurposeBacterial expression of 3C protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.DepositorInsertCVB3 3C protease with HisTag
Tags6xHis-tagExpressionBacterial, Insect, and Mamm…Promoterpolh, CAG, T7Available SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
GA46
Plasmid#196614PurposePlasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeastDepositorInsertATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac FAK W266A
Plasmid#186144PurposeFor expresseion of recombinant FAK using baccolovirus in SF9 cellsDepositorInsertPTK2 (PTK2 Mouse)
TagsHis6ExpressionInsectMutationDNA mutation T796G;G797C Amino acid mutation W266APromoterpolyhedrin promoterAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-FLAG-CBPΔ(N+HAT)
Plasmid#182841PurposeAn EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction.DepositorInsertCBP (w Fly)
TagsFLAG tagExpressionInsectMutationdletion of residues 1566-2142 from CBP1476-3222PromoterMTAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TEV CHMP1B 186-199 L188A/L192A
Plasmid#108288PurposeExpresses residues 186-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-286DepositorInsertCHMP1B (CHMP1B Human)
TagsGSTExpressionBacterialMutationchanged L 188 to A, changed L 192 to AAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TEV CHMP1B 186-199 L192A/L195A
Plasmid#108290PurposeExpresses residues 186-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-287DepositorInsertCHMP1B (CHMP1B Human)
TagsGSTExpressionBacterialMutationchanged L 188 to A, changed L 192 to AAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Flag-Rheb-12Rs
Plasmid#165023Purposeexpression of Flag-Rheb-12Rs mutant protein in mammalian cells (all the lysine residues on Rheb except the K19 and K120 are mutated to arginines).DepositorInsertRheb-12Rs (RHEB Human)
UseLentiviralTagsflagExpressionMammalianMutationAll the lysine residues except K19 and K120 are c…Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-H253A-R256A-R260A
Plasmid#127788PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in His2…Available SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-GFP-I3(resist)
Plasmid#61400PurposeExpresses GFP-tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cellsDepositorInsertInhibitor-3 (PPP1R11 Human)
TagsGFPExpressionMammalianMutationsilent mutations of bases C183T T192C T189CPromoterCMVAvailable SinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
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