We narrowed to 10,808 results for: T7
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Plasmid#163128PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding MalE signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only
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pLyGo-Ec-5
Plasmid#163131PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
OfCasp3a
Plasmid#166053Purposea caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but has CARD domain and function as an initiator caspaseDepositorInsertOrbicella faveolata caspase-3a
TagsHis tagExpressionBacterialPromoterT7 lac promoterAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
PaCasp7a
Plasmid#166051Purposea caspase of Porites astreoides, which has higher sequence similarity with human caspase-7 but has a CARD domain and function as an initiator caspaseDepositorInsertPorites astreiodes caspase-7a
TagsHis tagExpressionBacterialPromoterT7 promoterAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSF-ChPylTMSK
Plasmid#163915PurposetRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK), into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT047A
Plasmid#159408PurposeConstitutive LuxR expression, controlled under T7 bacteriophage promoter, and inducible PFO-sfGFP chimeric protein expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.T7-LuxR-pr.LuxR-sfGFP-PFO
UseSynthetic BiologyTagsFLAG/FLAGExpressionBacterialPromotertet and pLux promoterAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGST-Fah_S
Plasmid#163088Purposeexpressing mouse swingshift mutant FAH in bacterial cellsDepositorInsertFah_S
TagsGSTExpressionBacterialPromoterT7Available SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
AlkB-D135G
Plasmid#160434PurposeA plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to GlyDepositorInsertE. coli AlkB
TagsHis-tagExpressionBacterialMutationChanged Aspartic acid 135 to Glycine; deleted the…PromoterT7Available SinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1HR1AtR2NtB2
Plasmid#160890PurposeExpression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-S1H
Tags6xHisExpressionBacterialPromoterrbcS-SH1: T7, chaperones: T13Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT4aR1AtR2NtB2
Plasmid#160887PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T4a
ExpressionBacterialPromoterrbcS-T4a: T7, chaperones: T10Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pET15-His-∆NGlpG(Y205F)
Plasmid#155132PurposeExpresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
TagsHisExpressionBacterialMutationY205F, insert codes for GlpG residues 87-276PromoterT7Available SinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS
Plasmid#153443PurposeE. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.DepositorInsertRc.XDocB
Tags6xHis and ybbr tagExpressionBacterialMutationSerine 199 was mutated to amber codon (TAG)PromoterT7 promoterAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz172_CsoS2_N1_GFP
Plasmid#140852PurposeHis-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backboneDepositorInsertCsoS2 NTD repeat 1 fused to GFP
TagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialPromoterT7Available SinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz175_CsoS2_N4_GFP
Plasmid#140855PurposeHis-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 4 fused to GFP
TagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialPromoterT7Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz173_CsoS2_N2_GFP
Plasmid#140853PurposeHis-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 2 fused to GFP
TagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialPromoterT7Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz174_CsoS2_N3_GFP
Plasmid#140854PurposeHis-tagged third N-peptide of NTD fused to Superfolder GFP ([N3]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 3 fused to GFP
TagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialPromoterT7Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAWH-IRP
Plasmid#133896PurposePositive control when used with pMS22H-IRE. Expression of Gal4AD-IRP hybrid protein. Homology regions for recombination with pMS22HDepositorInsertIRP
ExpressionYeastPromoterT7Available SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK_ToeRepG2_N64_trigger
Plasmid#132746PurposeT7 RNAP-driven expression of second-generation toehold repressor index 64 trigger RNADepositorInsertToeRepG2_N64_trigger
ExpressionBacterialAvailable SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only