We narrowed to 19,051 results for: Tec;

1. pOCC254

   Plasmid

   #118889

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6-MBP, cleavable with 3C protease; mCherry, cl…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTox-EGFPsite1_NGA-PAM (BPK754)

   Plasmid

   #181747

   Purpose

   p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGA PAM

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   ccdB toxin gene and SpCas9 target site (EGFP site 1 NGA PAM)
   

   Use

   Selection plasmid

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. mEos2-Talin-IBS2-7

   Plasmid

   #57424

   Purpose

   Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

   Insert

   Talin IBS2 (Tln1 Mouse)
   

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Talin Integrin Binding Site

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pRSETa-Amrose v1

   Plasmid

   #79537

   Purpose

   pRSETa (Invitrogen) plasmid expressing Amrose variant 1

   Depositor

   Randolph Caldwell

   Article

   Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

   Insert

   Amrose
   

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPTXcGMPRELUC

   Plasmid

   #68503

   Purpose

   cyclic nucleotide reporter for bacteria or plant cells

   Depositor

   Helen Irving

   Article

   Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

   Insert

   OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
   

   Tags

   luciferase

   Expression

   Bacterial and Plant

   Mutation

   3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…

   Promoter

   OPTX promoter with cGMPRE

   Available Since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

   Plasmid

   #160610

   Purpose

   Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   P35s:AsCas12a:T35s
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. UASgal-A9-Pleum

   Plasmid

   #60343

   Purpose

   Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.

   Depositor

   Hal Alper

   Article

   Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

   Insert

   promoter
   

   Use

   Synthetic Biology; Yeast synthetic hybrid promote…

   Expression

   Yeast

   Available Since

   Nov. 6, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCfB3053(gRNA X-2, XI-5, XII-4)

   Plasmid

   #73295

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Zebrafish-gRNA-0001

   Plasmid

   #42241

   Purpose

   gRNA targeted to zebrafish gene apoea

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   gRNA-apoea (apoea Zebrafish)
   

   Use

   CRISPR; Zebrafish expression

   Promoter

   T7

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

10. mKO-Fibrillarin-7

    Plasmid

    #57842

    Purpose

    Localization: Nuclear - Nucleolus, Excitation: 548, Emission: 561

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Fibrillarin (FBL Human)
    

    Tags

    mKO

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMDC32-FIT2

    Plasmid

    #96990

    Purpose

    Express mouse FIT2 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

    Insert

    MmFIT2 (Fitm2 Mouse)
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCTCON2-BXG-2TAG

    Plasmid

    #158138

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the first serine residue…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)

    Plasmid

    #160635

    Purpose

    TU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminator

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

    Insert

    AcrIIA4
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-dLbCpf1-BE0ΔiNLS

    Plasmid

    #107684

    Purpose

    Expresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE0ΔiNLS
    

    Expression

    Mammalian

    Mutation

    D832A/E925A/D1148A

    Promoter

    CMV

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUPD2 AtU6-26:LbCas12a_DR (GB1443)

    Plasmid

    #160575

    Purpose

    LbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    AtU6-26:LbCas12a_DR
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    U6-26

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB3038(XII-1 MarkerFree)

    Plasmid

    #73278

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB3040(XII-4 MarkerFree)

    Plasmid

    #73280

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPK1E-1-gfp

    Plasmid

    #48133

    Purpose

    Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    gfp
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. TAL2317

    Plasmid

    #36743

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FGFR1-TALEN_Right (FGFR1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

20. TAL2316

    Plasmid

    #36742

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FGFR1-TALEN_Left (FGFR1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only