We narrowed to 24,314 results for: crispr

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pFETCh_NFIL3

Plasmid

#86287

Purpose

Donor vector for 3' FLAG tag of human NFIL3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

NFIL3 homology arms (NFIL3 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

Plasmid

#133340

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_gcrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dHgm4Cas13b-EGFP-NLS

Plasmid

#191360

Purpose

Overexpression of NLS-dHgm4Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dHgm4Cas13b-EGFP-NLS

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

Plasmid

#133334

Purpose

for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pM116: CasRx VEGFA presgRNA

Plasmid

#166868

Purpose

U6-driven expression of human VEGFA targeting presgRNA compatible with CasRx.

Depositor

Guei-Sheung Liu

Article

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Insert

Human VEGFA targeting presgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pET-3a-His-MBP-TEV-VC_Cas1_WT

Plasmid

#120364

Purpose

Expresses wildtype V-C Cas1

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

VC_Cas1

Use

CRISPR

Expression

Bacterial

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET-3a-His-MBP-TEV-VD_Cas1_WT

Plasmid

#120365

Purpose

Expresses wildtype V-D Cas1

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

VD_Cas1

Use

CRISPR

Expression

Bacterial

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ141C2.0

Plasmid

#99905

Purpose

Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFETCh_MIXL1

Plasmid

#86280

Purpose

Donor vector for 3' FLAG tag of human MIXL1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MIXL1 homology arms (MIXL1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_TEAD3

Plasmid

#86281

Purpose

Donor vector for 3' FLAG tag of human TEAD3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

TEAD3 homology arms (TEAD3 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pICSL11023

Plasmid

#117542

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)

Expression

Plant

Promoter

2x35S_OMEGA(pICH51288)

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pFETCh_NR2F6

Plasmid

#86284

Purpose

Donor vector for 3' FLAG tag of human NR2F6

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

NR2F6 homology arms (NR2F6 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_NCoA2

Plasmid

#86283

Purpose

Donor vector for 3' FLAG tag of human NCoA2

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

NCoA2 homology arms (NCOA2 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro

Plasmid

#192681

Purpose

Lentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Mouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_PRKRA

Plasmid

#106109

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting PRKRA

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting PRKRA (PRKRA Human)

Use

CRISPR

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-rpsL

Plasmid

#89953

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rpsL.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

rpsL gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEJS507-pCDest2-noAcr-mTagBFP2-IRES

Plasmid

#85748

Purpose

Expresses mTagBFP2 only; used as a control

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

mTagBFP2

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDIV490

Plasmid

#177698

Purpose

Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RHR2p (Debaryomyces hansenii ) and TDH3p (Candida…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-BsCas12b-2AeGFP

Plasmid

#121948

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

BsCas12b

Tags

3xHA tag

Expression

Mammalian

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pFETCh_MLX

Plasmid

#86277

Purpose

Donor vector for 3' FLAG tag of human MLX

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MLX homology arms (MLX Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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