An educational module to explore CRISPR technologies with a cell-free transcription-translation system.
Collias D, Marshall R, Collins SP, Beisel CL, Noireaux V
Synth Biol (Oxf). 2019 Jan 21;4(1):ysz005. doi: 10.1093/synbio/ysz005. eCollection 2019. PubMed Article
Collias D, Marshall R, Collins SP, Beisel CL, Noireaux V
Synth Biol (Oxf). 2019 Jan 21;4(1):ysz005. doi: 10.1093/synbio/ysz005. eCollection 2019. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 119144 | pColE1_sgRNA_NT_AmpR | Encodes non-targeting sgRNA |
| 119145 | pColE1_sgRNA_pos2_AmpR | Encodes sgRNA targeting position 2 in pColE1_70a_deGFP_KanR |
| 119146 | pColE1_sgRNA_pos3_AmpR | Encodes sgRNA targeting position 3 in pColE1_70a_deGFP_KanR |
| 119148 | pColE1_sgRNA_pos5_AmpR | Encodes sgRNA targeting position 5 in pColE1_70a_deGFP_KanR |
| 119149 | pColE1_sgRNA_pos6_AmpR | Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR |
| 119150 | pColE1_sgRNA_pos7_AmpR | Encodes sgRNA targeting position 7 in pColE1_70a_deGFP_KanR |
| 119151 | pColE1_sgRNA_pos8_AmpR | Encodes sgRNA targeting position 8 in pColE1_70a_deGFP_KanR |
| 119152 | pColE1_sgRNA_pos9_AmpR | Encodes sgRNA targeting position 9 in pColE1_70a_deGFP_KanR |
| 119153 | pColE1_sgRNA_M1S_AmpR | Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the seed region |
| 119154 | pColE1_sgRNA_M2S_AmpR | Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed region |
| 119155 | pColE1_sgRNA_M1NS_AmpR | Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed region |
| 119156 | pColE1_sgRNA_M4NS_AmpR | Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with four mismatches in the non-seed region |
| 119157 | pColE1_sgRNA_M7NS_AmpR | Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region |
| 119158 | pColE1_sgRNA_T1_AmpR | Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end |
| 119159 | pColE1_sgRNA_T2_AmpR | Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end |
| 119160 | pColE1_sgRNA_T3_AmpR | Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end |
| 119161 | pColE1_70a_deGFP_AmpR | Expresses deGFP from a strong sigma 70 promoter |
| 119162 | p15A_SpyCas9_CmR | Expresses S. pyogenes Cas9 |
| 119163 | p15A_J23108_SpydCas9_CmR | Expresses S. pyogenes dCas9 |