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Vincent Noireaux Lab: An educational module to explore CRISPR technologies with a cell-free transcription-translation system
Unpublished

Plasmids from Article

ID Plasmid Purpose  
119144pColE1_sgRNA_NT_AmpREncodes non-targeting sgRNA
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119145pColE1_sgRNA_pos2_AmpREncodes sgRNA targeting position 2 in pColE1_70a_deGFP_KanR
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119146pColE1_sgRNA_pos3_AmpREncodes sgRNA targeting position 3 in pColE1_70a_deGFP_KanR
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119148pColE1_sgRNA_pos5_AmpREncodes sgRNA targeting position 5 in pColE1_70a_deGFP_KanR
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119149pColE1_sgRNA_pos6_AmpREncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR
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119150pColE1_sgRNA_pos7_AmpREncodes sgRNA targeting position 7 in pColE1_70a_deGFP_KanR
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119151pColE1_sgRNA_pos8_AmpREncodes sgRNA targeting position 8 in pColE1_70a_deGFP_KanR
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119152pColE1_sgRNA_pos9_AmpREncodes sgRNA targeting position 9 in pColE1_70a_deGFP_KanR
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119153pColE1_sgRNA_M1S_AmpREncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the seed region
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119154pColE1_sgRNA_M2S_AmpREncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed region
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119155pColE1_sgRNA_M1NS_AmpREncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed region
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119156pColE1_sgRNA_M4NS_AmpREncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with four mismatches in the non-seed region
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119157pColE1_sgRNA_M7NS_AmpREncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region
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119158pColE1_sgRNA_T1_AmpREncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end
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119159pColE1_sgRNA_T2_AmpREncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end
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119160pColE1_sgRNA_T3_AmpREncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end
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119161pColE1_70a_deGFP_AmpRExpresses deGFP from a strong sigma 70 promoter
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119162p15A_SpyCas9_CmRExpresses S. pyogenes Cas9
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119163p15A_J23108_SpydCas9_CmRExpresses S. pyogenes dCas9
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