Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 16,084 results for: nes

Clear keywords
Showing: 7381 - 7400 of 16084 results

  1. pET-FLAG-eSpCas9-plus

    Plasmid
    #126774
    Purpose
    Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126774-plasmid-map-sequence-id-410603

    Enlarge

  3. pSA073 HBB_IVS2-Cas9-BFP

    Plasmid
    #249134
    Purpose
    Overexpression of SpCas9-BFP with HBB IVS2 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    HBB_IVS2-Cas9-TagBFP (HBB Human, S. pyogenes Cas9, synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249134-plasmid-map-sequence-id-496493

    Enlarge

  8. pvPE-V3

    Plasmid
    #240287
    Purpose
    Encodes third generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.
    Depositor
    Lusheng Huang
    Article
    Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779
    Insert
    pvPE-V3
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240287-plasmid-map-sequence-id-488808

    Enlarge

  9. pUb-Cas9-mCherry_cU6:6-sgRNA

    Plasmid
    #190598
    Purpose
    The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter
    Depositor
    Claudia Rueckert
    Article
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    mCherry - separated by T2A
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available Since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190598-plasmid-map-sequence-id-379953

    Enlarge

  15. dCas9-CBP HAT

    Plasmid
    #179547
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179547-plasmid-map-sequence-id-354768

    Enlarge

  16. pPPC010.AAV

    Plasmid
    #171175
    Purpose
    Expression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Inserts
    hAAVS1 scRNA
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    BBa_J23119 and Sp.pCas9, BBa_J23107
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    171175-plasmid-map-sequence-id-344748

    Enlarge

Showing: 7381 - 7400 of 16084 results