We narrowed to 16,623 results for: puromycin

1. pSLQ7535 pHR (hU6-crSURF2-EFS-PuroR-WPRE)

   Plasmid

   #214879

   Purpose

   Lentiviral vector encoding RfxCas13d targeting SURF2 guide array

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Insert

   hU6-crSURF2-EFS-PuroR-WPRE
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCherryGFP-CoV2-WT

   Plasmid

   #177618

   Purpose

   Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting

   Depositor

   Junjie Guo

   Article

   Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

   Insert

   SARS-CoV-2 FSE
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDB_054

   Plasmid

   #216096

   Purpose

   All-in-one, knockout, delivers Cas12a & gRNA

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas12a [EnAs]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-N-Puro-mStayGold-LMNB1

   Plasmid

   #227329

   Purpose

   Donor template for Puro-2A-mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Puro-2A-mStayGold (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #161927

   Purpose

   Donor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

   Insert

   Surface marker and Citrine repression reporter
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   pEF

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-p300

   Plasmid

   #191764

   Purpose

   Expression of EGFP fused to core p300 histone acetyltransferase

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   FRB-EGFP-p300 core (EP300 Human)
   

   Tags

   EGFP (N-terminal of p300 core) and FRB (N-termina…

   Expression

   Bacterial and Mammalian

   Promoter

   EF1alpha

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSH-EFIRES-P-Seipin-miniIAA7-mCherry

   Plasmid

   #129720

   Purpose

   AAVS1 safe harbor targeting vector expressing miniIAA7-mCherry tagged Seipin

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   Seipin (BSCL2 Human)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   miniIAA7-mCherry

   Expression

   Mammalian

   Mutation

   silent mutations

   Promoter

   EF1a

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. paGFP-Omp25

   Plasmid

   #69598

   Purpose

   expresses GFP-labeled mitochondrial outer membrane protein 25

   Depositor

   David Sabatini

   Article

   Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

   Insert

   paGFP-Omp25 (Synj2bp Rat)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   photoactivatable GFP fused to rat Omp25 C-terminus

   Promoter

   CMV

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. TPC1-mCherry

   Plasmid

   #135182

   Purpose

   Expresses TPC1 with C-terminus mCherry tag in mammalian cells

   Depositor

   Antony Galione

   Article

   Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

   Insert

   TPC1 (Tpcn1 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pScalps_Puro_mCebpa

    Plasmid

    #79551

    Purpose

    Expression of mouse Cebpa

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Insert

    Cebp alpha (Cebpa Mouse)
    

    Use

    Lentiviral

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMKO.1 puro p53 shRNA 1

    Plasmid

    #10671

    Depositor

    William Hahn

    Article

    Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

    Insert

    p53 shRNA (TP53 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF2848

    Plasmid

    #144249

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF21 (TCF21 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX317-VRK1

    Plasmid

    #199643

    Purpose

    Expresses VRK1

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Lentiviral

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF0169

    Plasmid

    #141540

    Purpose

    Lentiviral vector for overexpressing the SIX3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SIX3 (SIX3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF2474

    Plasmid

    #144029

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ONECUT3 (ONECUT3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. RNASEL gRNA (BRDN0001149212)

    Plasmid

    #77051

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. RNASEL gRNA (BRDN0001149358)

    Plasmid

    #77052

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. GUT BuGZ FL

    Plasmid

    #84024

    Purpose

    Expression of GFP tagged BuGZ in human cells

    Depositor

    Patrick Paddison

    Article

    Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

    Insert

    BuGZ (ZNF207 Human)
    

    Use

    Lentiviral

    Tags

    TurboGFP

    Expression

    Mammalian

    Mutation

    Q394R

    Promoter

    UCOE / SFFV

    Available Since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pgRNA-CGG

    Plasmid

    #108248

    Purpose

    Lentiviral vector to express sgRNA targeting "GGCGGCGGCGGCGGCGGCGG" locus by SpCas9

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

    Insert

    sgRNA targeting GGCGGCGGCGGCGGCGGCGG
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-3FLAG-RAB11A

    Plasmid

    #176489

    Purpose

    For lentiviral transduction of 3FLAG-RAB11A

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    RAB11A (RAB11A Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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