We narrowed to 10,238 results for: ada

1. pNOC_dCas9_sgRNA Td2

   Plasmid

   #176258

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   dead spCas9
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Mutation

   H840A and D10A mutations on spCas9 to inactivate …

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNOC_dfnCas12a_crRNA Td2

   Plasmid

   #176255

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Mutation

   D917A and E1006A mutations to inactivate the endo…

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKHH030_sgCtrl#2

   Plasmid

   #174143

   Purpose

   Lentiviral vector expressing a control sgRNA that targets a safe harbor site

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSUMO GLTP

   Plasmid

   #170732

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

   Insert

   GLTP (GLTP Human)
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. deltaVP1 + VP2C-GFP-MIOX

   Plasmid

   #166682

   Purpose

   Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Inserts

   VP2C-GFP-MIOX
   Murine polyomavirus deltaVP1 (NLS deletion mutant)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   GAL1 and GAL10

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. MIOX only

   Plasmid

   #166681

   Purpose

   Yeast integrative plasmid for expressing mouse myo-inositol oxygenase (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Insert

   MIOX (Miox Budding Yeast, Synthetic, Mouse)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   GAL10

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. wtVP1 + VP2C-GFPdeg

   Plasmid

   #166678

   Purpose

   Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Inserts

   VP2C-GFPdeg
   Wild-type Murine polyomavirus VP1
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   GAL1 and GAL10

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. deltaVP1 + VP2C-GFPdeg

   Plasmid

   #166677

   Purpose

   Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Inserts

   VP2C-GFPdeg
   Murine polyomavirus deltaVP1 (NLS deletion mutant)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   GAL1 and GAL10

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Donor-CD55-NeoR

   Plasmid

   #153551

   Purpose

   Used as a donor vector for the targeted knock-in of a CD55 mutation. This plasmid carries a neomycin resistance gene cassette

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   mutant CD55 (a 1415-bp fragment from intron 3, exon 4 and intron 4, with a 1-bp mutation in exon 4), divided into 5' and 3' arms
   

   Use

   AAV and Cre/Lox; Donor plasmid/targeting vector

   Tags

   N/A

   Mutation

   a 1-bp mutation in exon 4

   Promoter

   No promoter

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Donor-PIGA-NeoR

    Plasmid

    #153549

    Purpose

    Used as a donor vector for the targeted knock-in of a PIGA mutation. This plasmid carries a neomycin resistance gene cassette

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6), divided into 5' and 3' arms
    

    Use

    AAV and Cre/Lox; Donor plasmid/targeting vector

    Tags

    N/A

    Mutation

    a 3-bp mutation in exon 6

    Promoter

    No promoter

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPR v2-Nr4a1sgRNA2

    Plasmid

    #160946

    Purpose

    Guide RNA 2 to generate Nr4a1 knockout by CRISPR

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Nr4a1 gRNA 2 (Nr4a1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Promoter

    hU6

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. DHC1-mIAA17 Hygro

    Plasmid

    #140544

    Purpose

    DHC1 tagging with mIAA7

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. px335-sgRNA-EF(Cas9n)-mHdac1-L2

    Plasmid

    #122307

    Purpose

    Expresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Hdac1 (Hdac1 Synthetic)
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-chiRNA_dCrkgRNA1

    Plasmid

    #131139

    Purpose

    Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    dCrk gRNA1 (Crk Fly)
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-chiRNA_dCrkgRNA2

    Plasmid

    #131140

    Purpose

    Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    dCrk gRNA1 (Crk Fly)
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1-TRC.mKO2_shHRASLS.2

    Plasmid

    #110324

    Purpose

    TRCN0000002620 (Target GCGATAAGTACCGTTGAGTTT), silence human HRASLS gene and express monomeric Kusabira-Orange2

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    HRASLS (PLAAT1 Human, Homo sapiens)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1-TRC.mKO2_shHRASLS.1

    Plasmid

    #110323

    Purpose

    TRCN0000378630 (Target GATAAGTACCGTTGAGTTTG), silence human HRASLS gene and express monomeric Kusabira-Orange2

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    HRASLS (PLAAT1 Human, Homo sapiens)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKK-TEV-mAmber

    Plasmid

    #105792

    Purpose

    Expression of your protein of interest in fusion with non-absorbing, non-emitting Venus mutant at the C-terminus (cleavable by TEV). Negative control for FRET measurements (PMID: 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mAmber

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKK-mAmber-TEV

    Plasmid

    #105775

    Purpose

    Expression of your protein of interest in fusion with non-absorbing, non-emitting Venus mutant at the N-terminus (cleavable by TEV). Negative control for FRET measurements (PMID: 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mAmber-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLuc-Optop38i-lsm

    Plasmid

    #89747

    Purpose

    Expression of light-regulated p38 inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor MK3BD3-13F

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    Optop38i lit-state mutant
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    I539E mutation of AsLov2Jalpha

    Promoter

    CMV

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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