We narrowed to 166,390 results for: addgene

1. SCML2_3

   Plasmid

   #220878

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Article

   Nady et al J Mol Biol. 2012 Nov 9;423(5):702-18. do

   Insert

   SCML2 (SCML2 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pSCL475

   Plasmid

   #219503

   Purpose

   Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

   Insert

   Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
   

   Expression

   Yeast

   Promoter

   GAL7

   Available Since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57.1 WT1 +378 ATG

   Plasmid

   #217644

   Purpose

   Dox inducible WT1 cDNA - as transcribed from +378ATG (short isoform)

   Depositor

   Constanze Bonifer

   Article

   Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

   Insert

   WT1 short isoform (WT1 Human)
   

   Expression

   Mammalian

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57.1 Ex8 mut WT1

   Plasmid

   #217645

   Purpose

   Dox inducible WT1 cDNA with exon 8 mutation

   Depositor

   Constanze Bonifer

   Article

   Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

   Insert

   WT1 (WT1 Human)
   

   Expression

   Mammalian

   Mutation

   Frameshift exon 8

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57.1 Ex9 mut WT1

   Plasmid

   #217646

   Purpose

   Dox inducible WT1 cDNA with exon 9 mutation

   Depositor

   Constanze Bonifer

   Article

   Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

   Insert

   WT1 (WT1 Human)
   

   Expression

   Mammalian

   Mutation

   Frameshift exon 9

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBDP

   Plasmid

   #217827

   Purpose

   Stuffer plasmid with golden gate compatible bidirectional promoter

   Depositor

   Timothy Whitehead

   Article

   Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

   Insert

   Gal1/Gal10 Bidirectional Promoter
   

   Use

   Cloning vector

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNS49 (enAsCas12a-KRAB piggyBac)

   Plasmid

   #217335

   Purpose

   piggyBac expresion of enAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   enAsCas12a
   

   Use

   CRISPR

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   E174R/S542R/K548R

   Promoter

   CAG

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-mKO2-Parkin

   Plasmid

   #213544

   Purpose

   Transient mammalian expresssion of Parkin, N-terminally tagged with mKO2, M1 of Parkin intact

   Depositor

   Steven Altschuler, Lani Wu

   Article

   Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

   Insert

   Parkin (PRKN Human)
   

   Tags

   mKO2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Parkin(E16A)

   Plasmid

   #213546

   Purpose

   Transient mammalian expresssion of E16A Parkin

   Depositor

   Steven Altschuler, Lani Wu

   Article

   Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

   Insert

   Parkin (PRKN Human)
   

   Expression

   Mammalian

   Mutation

   E16A mutation in Parkin

   Promoter

   CMV

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. LLP782_Traptavidin-KRAB-CO

    Plasmid

    #211775

    Purpose

    AviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SpyCatcher-DNMT3A
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available Since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LLP785_Traptavidin-DNMT3A-CO

    Plasmid

    #211778

    Purpose

    AviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Traptavidin-EZH2
    

    Tags

    3xV5

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect EZH2 e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. LLP781_SnoopCatcher-KRAB-CO

    Plasmid

    #211774

    Purpose

    SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Traptavidin-KRAB
    

    Tags

    3xV5

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. dCas9-RPT6-FLAG

    Plasmid

    #205416

    Purpose

    Expresses dCas9-RPT6 with FLAG label

    Depositor

    Timothy Jarome

    Article

    Farrell et al J Neurosci. 2024 Jan 24;44(4):e145323202

    Insert

    dCas9-RPT6 (Psmc5 Rat)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. phROSA26-Tet-HA-JLP_V55Q

    Plasmid

    #208771

    Purpose

    Tetracycline inducible expression vector for HA-JLP_V55Q mutant protein, used as a donor plasmid for HA-JLP_V55Q knock-in at the human ROSA26 locus

    Depositor

    Katsuji Yoshioka

    Article

    Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

    Insert

    JLP (SPAG9 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA

    Plasmid

    #174897

    Purpose

    ochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sf GFP
    

    Expression

    Mammalian

    Mutation

    150TAA in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA

    Plasmid

    #174898

    Purpose

    opal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TGA in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFSynW pHluorin-SYT9 IRES mRuby3

    Plasmid

    #195698

    Purpose

    Lentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Rat)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFSynW Syt9 IRES mRuby3

    Plasmid

    #195700

    Purpose

    Lentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Lentiviral

    Mutation

    encodes only the first 68 amino acids of Tac1

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFSynW SYT9 D197, 199N IRES GFP

    Plasmid

    #195701

    Purpose

    Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Lentiviral

    Mutation

    full-length mouse SYT9 with D197, 199N mutations

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFSynW SYT9 D330, 332N IRES GFP

    Plasmid

    #195702

    Purpose

    Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Lentiviral

    Mutation

    full-length mouse SYT9 with D330, 332N mutations

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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