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pTn7-SCOUT22.RY

Plasmid

#201018

Purpose

pLv1-pUC18R6KT-miniTn7-Km-mCherry-sYFP2, KmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

mcherry
sYFP2

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT22.YB

Plasmid

#201019

Purpose

pLv1-pUC18R6KT-miniTn7-Km-sYFP2-TagBFP, KmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sYFP2
TagBFP

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-GST-YAP

Plasmid

#33052

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP1 (YAP1 Human)

Tags

GST

Expression

Mammalian

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pKm-HGBE

Plasmid

#170334

Purpose

a modularized single-plasmid glycosylase base editing (C-to-G) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized GBEs

Use

CRISPR

Mutation

Cas9n-D10A

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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SM282_EF1A-dEnAsCas12a-P300-T2A-BlastR

Plasmid

#244217

Purpose

Mammalian expression of dEnAsCas12a-P300 with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a-P300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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FCHo2-pmCherryC1

Plasmid

#27686

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

FCHo2 (Fcho2 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pKm-HABE

Plasmid

#170332

Purpose

a modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized ABEs

Use

CRISPR

Mutation

Cas9n-D10A

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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BIN1-pmCherryN1

Plasmid

#27693

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

BIN1 (Bin1 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
pBAD-Citron1

Plasmid

#134300

Purpose

Bacterial expression of direct-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citron1

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE503

Plasmid

#153276

Purpose

Intermediate sgRNA array cloning vector position 1

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE504

Plasmid

#153277

Purpose

Intermediate sgRNA array cloning vector position 2

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen

Plasmid

#182413

Purpose

Bacterial expression of mNeongreen

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-T7-OmpA-MNaseA-6xHis

Plasmid

#214808

Purpose

Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

Depositor

Peter Sarin

Article

Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

Insert

OmpA-Micrococcal nuclease A

Tags

6xhis

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET30-R5-GFP

Plasmid

#17838

Depositor

Jay Keasling

Article

Shaikh et al Anal Chem. 2008 Feb 1. 80(3):886-90.

Insert

pET30-GFP

Use

Synthetic Biology

Tags

His, R5, and S-tag

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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LaM8-GG15-irFP

Plasmid

#159591

Purpose

Opto-Nanobody

Depositor

Jose Avalos, Jared Toettcher

Article

Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

Insert

LaM8 with AsLOV2 at GG15

Use

Lentiviral

Available Since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pHCMV-RabiesG

Plasmid

#15785

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

Rabies virus G protein (RABVgp4 Rabies virus (strain SAD B19))

Expression

Mammalian

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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NM1-5S-tRNA-SgH

Plasmid

#128178

Purpose

Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

gRNA cloning cassette

Use

CRISPR

Expression

Yeast

Promoter

5S rRNA-tRNA(Arg)

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR1K18ms

Plasmid

#72644

Purpose

Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

Depositor

Brian Kvitko

Article

Mijatovic et al Phytopathology. 2021 Dec;111(12):2176-21

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available Since

March 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pG1

Plasmid

#162602

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pC28-LIC-sfGFP-His

Plasmid

#165479

Purpose

pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

TEV-GFP-6xHis

Expression

Bacterial

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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