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We narrowed to 8,588 results for: construct

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Showing: 7461 - 7480 of 8588 results

  3. pOPINB-hRNF216(457-784)

    Plasmid
    #171922
    Purpose
    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic helix-RBR-helix construct suitable for activity assays.
    Depositor
    Bernhard Lechtenberg
    Article
    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d
    Insert
    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    Tags
    3C protease cleavage site and 6x-His tag
    Expression
    Bacterial
    Mutation
    codon optimised for expression in E. coli
    Promoter
    T7
    Available Since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    171922-plasmid-map-sequence-id-346327

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  4. GPR123-DuET

    Plasmid
    #213262
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR123 (ADGRA1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213262-plasmid-map-sequence-id-430812

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  5. OPRK1-DuET

    Plasmid
    #213359
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPRK1 (OPRK1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213359-plasmid-map-sequence-id-430881

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  7. pCIBN-tagBFP-hTRF2

    Plasmid
    #103803
    Purpose
    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-tagBFP-hTRF2 (CIB1, TERF2 Mustard Weed, Human)
    Expression
    Mammalian
    Mutation
    hTRF2: deletion of amino acids 1-42 and 476 compa…
    Promoter
    CMV
    Available Since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103803-plasmid-map-sequence-id-199226

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  8. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid
    #171090
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…
    Mutation
    D9A, H599A
    Promoter
    TUB1 (Toxoplasma gondii), transcriptionally linke…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171090-plasmid-map-sequence-id-335786

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  9. CYSLTR1-DuET

    Plasmid
    #213225
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CYSLTR1 (CYSLTR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213225-plasmid-map-sequence-id-430787

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  10. GPR146-DuET

    Plasmid
    #213265
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR146 (GPR146 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213265-plasmid-map-sequence-id-430903

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  12. pOPINB-hRNF216(511-784)

    Plasmid
    #171923
    Purpose
    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic RBR-helix construct suitable for activity assays.
    Depositor
    Bernhard Lechtenberg
    Article
    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d
    Insert
    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    Tags
    3C protease cleavage site and 6x-His tag
    Expression
    Bacterial
    Mutation
    codon optimised for expression in E. coli
    Promoter
    T7
    Available Since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    171923-plasmid-map-sequence-id-346329

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  16. aCat DeltaTS (delta570 to 670 aa in aCat TS WT)

    Plasmid
    #101300
    Purpose
    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.
    Depositor
    Alpha Yap
    Article
    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.
    Insert
    alpha-Catenin
    Expression
    Mammalian
    Mutation
    Contains amino acids 1-509, 670-698, and 699-906 …
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    101300-plasmid-map-sequence-id-246103

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  18. CALCRL-DuET

    Plasmid
    #213194
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CALCRL (CALCRL Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    213194-plasmid-map-sequence-id-431751

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  19. CHRM3-DuET

    Plasmid
    #213209
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CHRM3 (CHRM3 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213209-plasmid-map-sequence-id-430771

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Showing: 7461 - 7480 of 8588 results