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pGal4-DBD-OCT4_AroPERFECT_N

Plasmid

#215624

Purpose

For overexpression of Gal4-DBD OCT4 AroPERFECT N

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

OCT4 AroPERFECT N

Expression

Mammalian

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGal4-DBD-OCT4_AroPERFECT_N

Plasmid

#215624

Purpose

For overexpression of Gal4-DBD OCT4 AroPERFECT N

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

OCT4 AroPERFECT N

Expression

Mammalian

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pColdI-GST-mGTSF2

Plasmid

#215714

Purpose

Bacterial expression of GST-tagged GTSF2

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

GTSF2 (Gtsf2 Mouse)

Tags

6x His and GST

Expression

Bacterial

Promoter

CspA

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pColdI-GST-mGTSF2

Plasmid

#215714

Purpose

Bacterial expression of GST-tagged GTSF2

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

GTSF2 (Gtsf2 Mouse)

Tags

6x His and GST

Expression

Bacterial

Promoter

CspA

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLVTHM-mSox17FNV-3xflag

Plasmid

#206375

Purpose

Expresses 3xflag fused mouse SOX17FNV in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17FNV-3xflag (Sox17 Mouse)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLVTHM-mSox17FNV-3xflag

Plasmid

#206375

Purpose

Expresses 3xflag fused mouse SOX17FNV in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17FNV-3xflag (Sox17 Mouse)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRset-rogRFP2

Plasmid

#207875

Purpose

Bacterial expression of the red fluorescent rogRFP2 redox sensor

Depositor

Mathew Tantama

Article

Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

Insert

rogRFP2

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRset-rogRFP2

Plasmid

#207875

Purpose

Bacterial expression of the red fluorescent rogRFP2 redox sensor

Depositor

Mathew Tantama

Article

Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

Insert

rogRFP2

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Shim_CytC_TP_GFP_pK7FWG2

Plasmid

#202646

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from putative Cytochrome C

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Shim_CytC_TP_GFP_pK7FWG2

Plasmid

#202646

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from putative Cytochrome C

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-THTAP-SPARTIN, residues 1-101

Plasmid

#180626

Purpose

Bacterial expression for SPARTIN MIT residues 1-101. Has N-terminal HIS-GST tag. Internal ID: WISP20-57.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPARTIN

Tags

HIS-GST

Expression

Bacterial

Mutation

Residues 1-101

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-THTAP-SPARTIN, residues 1-101

Plasmid

#180626

Purpose

Bacterial expression for SPARTIN MIT residues 1-101. Has N-terminal HIS-GST tag. Internal ID: WISP20-57.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPARTIN

Tags

HIS-GST

Expression

Bacterial

Mutation

Residues 1-101

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-THTAP-CALPAIN-7 1-165

Plasmid

#180610

Purpose

Bacterial expression vector for CALPAIN-7 residues 1-165. Has N-terminal cleavable HIS-GST tag.Internal ID: WISP21-129

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Tags

HIS-GST

Expression

Bacterial

Mutation

Residues 1-65

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165

Plasmid

#180611

Purpose

Bacterial expression vector for CALPAIN-7 residues 1-165, tandem MIT domains. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-25

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-THTAP-CALPAIN-7 1-165

Plasmid

#180610

Purpose

Bacterial expression vector for CALPAIN-7 residues 1-165. Has N-terminal cleavable HIS-GST tag.Internal ID: WISP21-129

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Tags

HIS-GST

Expression

Bacterial

Mutation

Residues 1-65

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165

Plasmid

#180611

Purpose

Bacterial expression vector for CALPAIN-7 residues 1-165, tandem MIT domains. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-25

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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JAB3479

Plasmid

#190417

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt

Expression

Plant

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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JAB3479

Plasmid

#190417

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt

Expression

Plant

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_B-Pho2-1/2-2-tRNA

Plasmid

#161761

Purpose

MODULE B tRNA vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2303 - #91068)

Depositor

Shaun Curtin

Article

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

gRNAs

Expression

Bacterial

Promoter

CmYLCV Promoter

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_B-Pho2-1/2-2-tRNA

Plasmid

#161761

Purpose

MODULE B tRNA vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2303 - #91068)

Depositor

Shaun Curtin

Article

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

gRNAs

Expression

Bacterial

Promoter

CmYLCV Promoter

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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