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pET-29b(+)_notufA-EmEndoF2-CBD-His

Plasmid

#218197

Purpose

Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF2

Depositor

Florence Pojer

Insert

EndoF2

Tags

CBD-His

Expression

Bacterial

Mutation

codon optimized Ecoli, residues 45-335

Promoter

T7

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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LZ3 dFE

Plasmid

#190144

Purpose

Expresses LZ3Cas9-T4pol (LZ3 dFE)

Depositor

Timothy Lu

Article

Nakade et al bioRxiv 2022.12.05.518807

Insert

LZ3Cas9-T4pol (LZ3 dFE)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Codon Optimized for mammalian cells

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Cas9_P2A_POLB

Plasmid

#190145

Purpose

Expresses SpCas9 and POLB

Depositor

Timothy Lu

Article

Nakade et al bioRxiv 2022.12.05.518807

Insert

SpCas9_P2A_POLB

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Codon Optimized for mammalian cells

Promoter

CBh

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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loxP-T2A-Myr-Casp8-ERT2-rox

Plasmid

#182362

Purpose

Shuttle vector for T2A-Myr-Casp8-ERT2

Depositor

Mario Capecchi

Insert

loxP-T2A-Myr-Casp8-ERT2-rox

Use

Cre/Lox and Mouse Targeting; Dre / rox

Expression

Mammalian

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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loxP-T2A-Myr-DeadCasp8-ERT2-rox

Plasmid

#182361

Purpose

Shuttle vector for T2A-Myr-DeadCasp8-ERT2

Depositor

Mario Capecchi

Insert

loxP-T2A-Myr-DeadCasp8-ERT2-rox

Use

Cre/Lox and Mouse Targeting; Dre / rox

Expression

Mammalian

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAM-35s-Snrk2.3-YFPn-1

Plasmid

#102391

Purpose

split YFP. Plant expression of Snrk2.3-YFPn-1

Depositor

Silke Robatzek

Insert

SNRK2.3 (SNRK2.3 Mustard Weed)

Tags

YFPn

Expression

Plant

Promoter

35S

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCM305

Plasmid

#44550

Depositor

Michael Grunstein

Article

Millar et al Genes Dev. 2006 Mar 15;20(6):711-22.

Insert

HA-htz1

Expression

Yeast

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
pPR244-Bic-C

Plasmid

#26139

Depositor

Phillip Newmark

Article

Wang et al Genes Dev. 2010 Sep 15. 24(18):2081-92.

Insert

Smed-Bicaudal-C

Use

RNAi

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pPR244-NF-YB

Plasmid

#26147

Depositor

Phillip Newmark

Article

Wang et al Genes Dev. 2010 Sep 15. 24(18):2081-92.

Insert

Smed-NF-YB

Use

RNAi

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pBS-NF-YB

Plasmid

#26136

Depositor

Phillip Newmark

Article

Wang et al Genes Dev. 2010 Sep 15. 24(18):2081-92.

Insert

Smed-NF-YB

Use

Cloning

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only
pYBS139

Plasmid

#18900

Depositor

Elaine Elion

Article

Kranz et al Genes Dev. 1994 Feb 1. 8(3):313-27.

Insert

FAR1 (FAR1 Budding Yeast)

Expression

Yeast

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut A

Plasmid

#11314

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation A (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut B

Plasmid

#11315

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation B (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut C

Plasmid

#11316

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation C (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut D

Plasmid

#11317

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation D (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut E

Plasmid

#11318

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation E (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut F

Plasmid

#11319

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation F (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut H

Plasmid

#11321

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation H (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut GFP

Plasmid

#11323

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 bulged binding sites for CXCR4 siRNA antisense, flanking 2 binding sites for GFP

Use

Luciferase

Tags

Rr-luc

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pTNS3

Plasmid

#63127

Purpose

Tn7 transposase expression

Depositor

Herbert Schweizer

Article

Choi et al Appl Environ Microbiol. 2008 Feb;74(4):1

Type

Empty backbone

Expression

Bacterial

Promoter

P1 and Ptac

Available Since

May 17, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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