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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,208 results for: grna

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Showing: 7561 - 7580 of 16208 results

  5. Cas9-sgRNA-A+B

    Plasmid
    #149370
    Purpose
    Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites
    Depositor
    Tarek Abbas
    Publication
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA-A
    sgRNA-B
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CBh, PGK, and U6
    Available since
    Oct. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    149370-plasmid-map-sequence-id-315474

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  6. pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

    Plasmid
    #164266
    Purpose
    sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.
    Depositor
    Nikolaus Rajewsky
    Publication
    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Worm
    Promoter
    U6 promoter from W05B2.8
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    164266-plasmid-map-sequence-id-325780

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  8. gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

    Plasmid
    #217662
    Purpose
    expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL
    Depositor
    Daniel Gerlich
    Publication
    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)
    Use
    CRISPR
    Expression
    Bacterial and Mammalian
    Promoter
    U6
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    217662-plasmid-map-sequence-id-440854

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  11. pX459-sgRNA2-RPB1-N-term

    Plasmid
    #195111
    Purpose
    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).
    Depositor
    Job Dekker
    Publication
    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA against first exon of RPB1 (N-terminal)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195111-plasmid-map-sequence-id-383881

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  13. pX335-NQL002-WAPL-sgRNA1

    Plasmid
    #175550
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL003-WAPL-sgRNA2 targeting construct.
    Depositor
    Elzo de Wit
    Publication
    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9-nickase and sgRNA against mouse WAPL STOP Codon
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    175550-plasmid-map-sequence-id-345078

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  14. pX335-NQL003-WAPL-sgRNA2

    Plasmid
    #175551
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL002-WAPL-sgRNA1 targeting construct.
    Depositor
    Elzo de Wit
    Publication
    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9-nickase and sgRNA against mouse WAPL STOP Codon
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    175551-plasmid-map-sequence-id-345081

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Showing: 7561 - 7580 of 16208 results