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pROF394

Plasmid

#155333

Purpose

Expression of PULSE-controlled LFY-VP16-2A-RLuc

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

(etr)8-(C120)5-PhCMVmin-LFY-VP16-NLS-2A-RLuc-TSV40

Use

Synthetic Biology; Eukaryotic expression

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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CoV2-N-M234I

Plasmid

#177955

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (M234I). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (M234I) (N )

Expression

Mammalian

Mutation

M234I

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-D377Y

Plasmid

#177957

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D377Y). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (D377Y) (N )

Expression

Mammalian

Mutation

D377Y

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-P13L

Plasmid

#177946

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P13L). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (P13L) (N )

Expression

Mammalian

Mutation

P13L

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-P80R

Plasmid

#177947

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (P80R) (N )

Expression

Mammalian

Mutation

P80R

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-S235F

Plasmid

#177956

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S235F). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (S235F) (N )

Expression

Mammalian

Mutation

S235F

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD ΔS/GFP

Plasmid

#253144

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of spike, GFP is inserted at the locus of Spike

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD ΔS/GFP

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_ORF6-BirA

Plasmid

#253132

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), BirA fused to orf6, C-terminal

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD_ORF6-BirA (ORF6 SARS-CoV-2)

Tags

BirA

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2_ΔE67 3*

Plasmid

#253186

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD2_ΔE67 ORF3*

Expression

Bacterial

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD1_XBB.1.5 S

Plasmid

#253119

Purpose

For recombinant virus rescue; encodes fragment D1 of SARS-CoV-2 (nt20950-25522), Omicron isolate XBB.1.5 Spike

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

SARS-CoV-2 Omicron XBB.1.5 spike

Expression

Bacterial

Mutation

S: F1089F

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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