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We narrowed to 860 results for: golden gate assembly

Showing: 741 - 760 of 860 results

  1. pCAG-T7-TALEN(Sangamo)-Destination

    Plasmid
    #37184
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.
    Depositor
    Pawel Pelczar
    Article
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Use
    Tags
    FokI nuclease
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 24, 2012
    Availability
    Academic Institutions and Nonprofits only
    37184-plasmid-map-sequence-id-46727

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  6. pNM3749

    Plasmid
    #154108
    Purpose
    loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector
    Depositor
    Michael Nonet
    Article
    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.
    Inserts
    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::his-58::FRT3
    Use
    Cre/Lox; Rmce
    Tags
    Expression
    Worm
    Mutation
    Promoter
    not applicable
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    154108-plasmid-map-sequence-id-306067

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  7. pLY134

    Plasmid
    #130962
    Purpose
    Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEB5
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Anderson promoter J23100
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    130962-plasmid-map-sequence-id-257714

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  17. pEPQD0KN0244

    Plasmid
    #162282
    Purpose
    Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis
    Depositor
    Nicola Patron
    Article
    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162282-plasmid-map-sequence-id-320851

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  18. pEPQD0KN0024

    Plasmid
    #162281
    Purpose
    Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis
    Depositor
    Nicola Patron
    Article
    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162281-plasmid-map-sequence-id-320849

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  19. pLY133

    Plasmid
    #130961
    Purpose
    Engineered sgRNA-LEB4 generator including Anderson promoter J23100 for golden gate assembly
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEB4
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Anderson promoter J23100
    Available since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    130961-plasmid-map-sequence-id-266878

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  20. pLY131

    Plasmid
    #130956
    Purpose
    Engineered sgRNA-LEB2 generator including Anderson promoter J23100 for golden gate assembly
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEB2
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Anderson promoter J23100
    Available since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    130956-plasmid-map-sequence-id-266875

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Showing: 741 - 760 of 860 results