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Showing: 741 - 760 of 834 results

  1. pLaG2-T7RNAPC

    Plasmid
    #216337
    Purpose
    Arabinose-inducible PBad promoter driving expression of the anti-eGFP nanobody LaG2 translationally fused to the C-terminal portion (residues 180-883) of T7RNAP
    Depositor
    Mark Styczynski
    Insert
    LaG2-T7RNAPC fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    g2658a, g3915a
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    216337-plasmid-map-sequence-id-456751

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  3. pVQ CMV NanoV1-2a-EGFP ferritin

    Plasmid
    #79649
    Purpose
    Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein
    Depositor
    Jeffrey Friedman
    Article
    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do
    Inserts
    transient receptor potential vanillin 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    79649-plasmid-map-sequence-id-175067

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  4. pVQ CMV NanoV1Mutant-2a-EGFP ferritin

    Plasmid
    #79650
    Purpose
    Expresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin chimera fusion protein
    Depositor
    Jeffrey Friedman
    Article
    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do
    Inserts
    transient receptor potential vanilloid 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    isoleucine 679 changed to lysine
    Promoter
    CMV
    Available since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    79650-plasmid-map-sequence-id-156910

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  5. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

    Plasmid
    #162877
    Purpose
    Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM
    Depositor
    Frederic Meunier
    Article
    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p
    Insert
    pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Insert consists of AA1-170
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    162877-plasmid-map-sequence-id-321985

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  8. XXBLM (XXBLMA-c075)

    Plasmid
    #98223
    Purpose
    Vector for periplasmic expression of Lama antibodies with PelA leader and C-terminal His6 tag
    Depositor
    Nicola Burgess-Brown
    Insert
    XXBLMA (BLM Human)
    Use
    Tags
    His-6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    98223-plasmid-map-sequence-id-193299

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  14. pJYDC3

    Plasmid
    #162460
    Purpose
    Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter DnbALFA was fused to the N-terminus of Aga2p.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    DnbALFA designed ALFA nanobody, HA tag, HDEL sequ…
    Expression
    Mutation
    Promoter
    Available since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162460-plasmid-map-sequence-id-330057

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  16. pFUSE-rabbitFc1-IL2ss-VHH(G08)-His-Myc

    Plasmid
    #209857
    Purpose
    Plasmid coding for a nanobody (termed G08) against FGFR3 (mus musculus) with x6 HisTag, x3 myc, and Fabbit FC gamma 1
    Depositor
    Tim Brown
    Inserts
    IL2-ss
    VHH (G08 variant)
    Rabbit Fc Gamma 1
    Use
    Tags
    x3 MycTag and x6 HisTag
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1-HTLV promoter
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    209857-plasmid-map-sequence-id-414817

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  20. pFUSE-rabbitFc1-IL2ss-VHH(B12)-His-Myc

    Plasmid
    #209837
    Purpose
    Plasmid coding for a nanobody against FGFR3 (mus musculus) with x6 HisTag, x3 myc, and Fabbit FC gamma 1
    Depositor
    Tim Brown
    Inserts
    IL2-ss
    VHH (B12 variant)
    Rabbit Fc Gamma 1
    Use
    Tags
    x3 MycTag and x6 HisTag
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1-HTLV promoter
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    209837-plasmid-map-sequence-id-414818

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Showing: 741 - 760 of 834 results