We narrowed to 10,315 results for: otos
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Plasmid#229434Purposeknockout of MARK2DepositorInsertsgMARK2-R-1 (MARK2 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterhU6;bU6;EFSAvailable SinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-YAP-5D
Plasmid#229415PurposeLentiviral or overpexression of cDNADepositorInsertYAP-5D (YAP1 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (S128,S163,S350,T354,T398) mut…PromoterEFSAvailable SinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-YAP-5A
Plasmid#229413PurposeLentiviral or overpexression of cDNADepositorInsertYAP-5A (YAP1 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (S128,S163,S350,T354,T398) mut…PromoterEFSAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-EFS (EF1a core) (JDW 1319)
Plasmid#224474PurposeGateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.DepositorInsertEFS promoter
ExpressionMammalianAvailable SinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)
Plasmid#224505PurposeGateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expressionDepositorInsertLexA-mODC-LexOP-35SCaMV
UseGateway cloningAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-LexA-mODC-LexOP-c-Fos (JDW 1359)
Plasmid#224506PurposeGateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression constructDepositorInsertLexA-oMDC-LexOP-c-Fos
UseGateway cloningAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)
Plasmid#224512PurposeA Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)DepositorInsert3xHA-BirA-T2A-mCherry-Caax
UseGateway cloningAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)
Plasmid#224522PurposeA Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassetteDepositorInsertlox-3xNLS-mCherry-V5-SV40pA-3xstop-lox
UseCre/LoxAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
mTph2p(330bp)-Luc
Plasmid#223663PurposeFirefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoterDepositorInsertmTph2 promoter (Tph2 Mouse)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianPromotermTph2 promoterAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIG-LSL-LRRC37B
Plasmid#220791PurposeTo express the human receptor LRRC37B (cre-dependant) in mammalian cells together with EGFPDepositorInsertLRRC37B (LRRC37B Human)
Available SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIG-LRRC37B-HA
Plasmid#220792PurposeTo express the human receptor LRRC37B HA-tagged in mammalian cells together with EGFPDepositorInsertLRRC37B (LRRC37B Human)
TagsHAAvailable SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-VIM270-pSM
Plasmid#215871PurposeThis plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.DepositorInsert3 tandem repeats of target site (complementary to seed seq of passenger strand of siRNA for VIM) is inserted to 3'UTR of RLuc (VIM Human)
ExpressionMammalianAvailable SinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-VIM270-pCM
Plasmid#215869PurposeThis plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.DepositorInserttarget site which is completely complementary to passanger strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
ExpressionMammalianAvailable SinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-749_HA-GD2-28z_CAR_TFAP4
Plasmid#207490PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertTFAP4, HA-GD2-28z_CAR (TFAP4 Human)
UseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-852_HA-GD2-28z_TFAP4-HA
Plasmid#207502PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertTFAP4-HA, HA-GD2-28z_CAR (TFAP4 Human)
UseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-858_CD19-BBz_CAR_TFAP4
Plasmid#207503PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertTFAP4, CD19-BBz_CAR (TFAP4 Human)
UseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ZIC3_5-3)-PGKpuroBFP-W
Plasmid#211996PurposeExpress gRNA against ZIC3 with puro and BFPDepositorInsertsgRNA targeting ZIC3 (ZIC3 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ZIC3_5-5)-PGKpuroBFP-W
Plasmid#211997PurposeExpress gRNA against ZIC3 with puro and BFPDepositorInsertsgRNA targeting ZIC3 (ZIC3 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(TRIM24_4)-PGKpuroBFP-W
Plasmid#211988PurposeExpress gRNA against TRIM24 with puro and BFPDepositorInsertsgRNA targeting TRIM24 (TRIM24 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(TRIM24_5-4)-PGKpuroBFP-W
Plasmid#211989PurposeExpress gRNA against TRIM24 with puro and BFPDepositorInsertsgRNA targeting TRIM24 (TRIM24 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only