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We narrowed to 7,661 results for: Pol

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Showing: 7621 - 7640 of 7661 results

  1. HUHe variant click editor (CE1) - pCMV-T7-CoCV-nCas9-EcKlenow (JO1262)

    Plasmid
    #217786
    Purpose
    Variant CE1 construct with CoCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    CoCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217786-plasmid-map-sequence-id-432849

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  2. HUHe variant click editor (CE1) - pCMV-T7-FiCV-nCas9-EcKlenow (JO1263)

    Plasmid
    #217787
    Purpose
    Variant CE1 construct with FiCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    FiCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217787-plasmid-map-sequence-id-432850

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  3. HUHe variant click editor (CE1) - pCMV-T7-GuCV-nCas9-EcKlenow (JO1279)

    Plasmid
    #217788
    Purpose
    Variant CE1 construct with GuCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    GuCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217788-plasmid-map-sequence-id-432851

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  4. HUHe variant click editor (CE1) - pCMV-T7-PCV1-nCas9-EcKlenow (JO1315)

    Plasmid
    #217789
    Purpose
    Variant CE1 construct with PCV1 HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV1-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217789-plasmid-map-sequence-id-432852

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  5. pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid
    #210736
    Purpose
    Coding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeats
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp D10A Cas9
    Merry sgCAG
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    D10A, N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available Since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210736-plasmid-map-sequence-id-420081

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  6. pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid
    #210735
    Purpose
    Coding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeats
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp D10A Cas9
    Gollum sgCAG
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    D10A, N terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available Since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210735-plasmid-map-sequence-id-420080

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  8. ATX3 13Q- I77K Q78K W87K

    Plasmid
    #185908
    Purpose
    Expresses ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-I77K, Q78K,W87K (N-terminal ) fused with His-Tag and TEV cleavage sequence (C-terminal).
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track and point mutations I77K, Q78K and W87K (ATXN3 Synthetic, Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Point mutations in Atx3 gene I77K Q78K and W87K
    Available Since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    185908-plasmid-map-sequence-id-370341

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  9. pMT-FLAG-CBPΔN

    Plasmid
    #182840
    Purpose
    A 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.
    Depositor
    Peter Harte
    Article
    Tie et al Development. 2009 Sep;136(18):3131-41. d
    Insert
    CBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)
    Tags
    FLAG tag
    Expression
    Insect
    Promoter
    MT
    Available Since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    182840-plasmid-map-sequence-id-360048

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  10. pGBW-m4046774

    Plasmid
    #145574
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145574-plasmid-map-sequence-id-287751

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  11. pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)

    Plasmid
    #79030
    Purpose
    Baculoviral entry vector to produce Bac-to-bac baculoviruses
    Depositor
    Wesley Sundquist
    Article
    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1
    Insert
    OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
    Tags
    OneSTrEP-FLAG
    Expression
    Insect
    Mutation
    deleted amino acid 294-515 from frican green monk…
    Promoter
    polyhedrin
    Available Since
    Jan. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    79030-plasmid-map-sequence-id-147998

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  12. Tf_dr3_1219ICCG4_b.46_h99_dd97_8

    Plasmid
    #257223
    Purpose
    Expression construct for Tf_dr3_1219ICCG4_b.46_h99_dd97_8 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    Tf_dr3_1219ICCG4_b.46_h99_dd97_8
    Expression
    Bacterial
    Mutation
    R18S, S19R, E27T, N28T, R46T, E47T, N48D, N49G, Y…
    Available Since
    July 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    257223-plasmid-map-sequence-id-517338

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  13. Tf_dr3_1159ICCG1_b.45_h99_dd97_7

    Plasmid
    #257220
    Purpose
    Expression construct for Tf_dr3_1159ICCG1_b.45_h99_dd97_7 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    Tf_dr3_1159ICCG1_b.45_h99_dd97_7
    Expression
    Bacterial
    Mutation
    R18T, S19R, S25A, E27T, N28T, R46T, E47S, N48T, N…
    Available Since
    July 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    257220-plasmid-map-sequence-id-517328

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  14. Tf_dr3_1219ICCG4_b.46_h99_dd97_7

    Plasmid
    #257221
    Purpose
    Expression construct for Tf_dr3_1219ICCG4_b.46_h99_dd97_7 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    Tf_dr3_1219ICCG4_b.46_h99_dd97_7
    Expression
    Bacterial
    Mutation
    R18S, S19R, E27T, N28T, R46T, E47T, N48E, N49G, Y…
    Available Since
    July 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    257221-plasmid-map-sequence-id-517332

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  15. Tf_dr3_1159ICCG5_b.45_h99_dd97_8

    Plasmid
    #257222
    Purpose
    Expression construct for Tf_dr3_1159ICCG5_b.45_h99_dd97_8 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    Tf_dr3_1159ICCG5_b.45_h99_dd97_8
    Expression
    Bacterial
    Mutation
    R18T, S19R, E26T, E27T, N28T, S35R, R46T, E47S, N…
    Available Since
    July 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    257222-plasmid-map-sequence-id-517335

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  16. Tf_dr3_1219ICCG1_b.46_h99_dd97_6

    Plasmid
    #257217
    Purpose
    Expression construct for Tf_dr3_1219ICCG1_b.46_h99_dd97_6 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    Tf_dr3_1219ICCG1_b.46_h99_dd97_6
    Expression
    Bacterial
    Mutation
    R18S, S19R, E27T, N28S, R46T, E47T, N48D, N49G, Y…
    Available Since
    July 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    257217-plasmid-map-sequence-id-517319

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  17. Tf_dr3_1219ICCG4_b.46_h99_dd97_6

    Plasmid
    #257218
    Purpose
    Expression construct for Tf_dr3_1219ICCG4_b.46_h99_dd97_6 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    Tf_dr3_1219ICCG4_b.46_h99_dd97_6
    Expression
    Bacterial
    Mutation
    R18S, S19R, S23A, E26Q, E27T, N28T, R46T, E47T, N…
    Available Since
    July 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    257218-plasmid-map-sequence-id-517322

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  18. Tf_dr3_1159ICCG5_b.45_h99_dd97_4

    Plasmid
    #257211
    Purpose
    Expression construct for Tf_dr3_1159ICCG5_b.45_h99_dd97_4 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    Tf_dr3_1159ICCG5_b.45_h99_dd97_4
    Expression
    Bacterial
    Mutation
    R18T, S19R, S23A, E26T, E27T, N28T, S35R, R46T, E…
    Available Since
    July 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    257211-plasmid-map-sequence-id-517304

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  19. Tf_dr3_1159ICCG5_b.45_h99_dd97_3

    Plasmid
    #257209
    Purpose
    Expression construct for Tf_dr3_1159ICCG5_b.45_h99_dd97_3 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    Tf_dr3_1159ICCG5_b.45_h99_dd97_3
    Expression
    Bacterial
    Mutation
    R18T, S19R, S23A, S25A, E26T, E27T, N28T, S35R, R…
    Available Since
    July 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    257209-plasmid-map-sequence-id-517298

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  20. Tf_dr3_1219ICCG1_b.46_h99_dd97_3

    Plasmid
    #257210
    Purpose
    Expression construct for Tf_dr3_1219ICCG1_b.46_h99_dd97_3 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    Tf_dr3_1219ICCG1_b.46_h99_dd97_3
    Expression
    Bacterial
    Mutation
    R18S, S19R, E27T, N28S, R46T, E47T, N48Q, N49G, Y…
    Available Since
    July 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    257210-plasmid-map-sequence-id-517300

    Enlarge

Showing: 7621 - 7640 of 7661 results