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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,352 results for: Cas9

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Showing: 7641 - 7660 of 14352 results

  1. pDONR221_SMAD2_HAe2_Bsd

    Plasmid
    #167166
    Purpose
    Blasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.
    Depositor
    Alexander Loewer
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Repair template to knockout SMAD2 by inserting a blasticidin resistance cassette (SMAD2 Human)
    Use
    Repair template for gene editing in human cells
    Tags
    blasticidin
    Available since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    167166-plasmid-map-sequence-id-330264

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  3. pEasyG3_mic

    Plasmid
    #184920
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Publication
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184920-plasmid-map-sequence-id-368413

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  6. pJEP15-AAV-H1/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82701
    Purpose
    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Publication
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82701-plasmid-map-sequence-id-157303

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  7. pJAT22

    Plasmid
    #204311
    Purpose
    For generating targeting inversions. N-terminal split GFP, expressed in eyes.
    Depositor
    David Stern
    Publication
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-GFPn
    Use
    CRISPR
    Promoter
    3XP3
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    204311-plasmid-map-sequence-id-465682

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  8. psBIG1a

    Plasmid
    #229957
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Publication
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    229957-plasmid-map-sequence-id-461574

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  9. psBIG1b

    Plasmid
    #229958
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Publication
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229958-plasmid-map-sequence-id-461279

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  10. psBIG2abc

    Plasmid
    #229963
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Publication
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229963-plasmid-map-sequence-id-461278

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  11. psBIG2ab

    Plasmid
    #229962
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Publication
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229962-plasmid-map-sequence-id-461276

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  12. psBIG2abcd

    Plasmid
    #229964
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Publication
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229964-plasmid-map-sequence-id-461270

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  13. psBIG2abcde

    Plasmid
    #229965
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Publication
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229965-plasmid-map-sequence-id-461268

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  14. psBIG1d

    Plasmid
    #229960
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Publication
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229960-plasmid-map-sequence-id-461267

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  15. psBIG1e

    Plasmid
    #229961
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Publication
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229961-plasmid-map-sequence-id-461266

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  16. psBIG1c

    Plasmid
    #229959
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Publication
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229959-plasmid-map-sequence-id-461263

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Showing: 7641 - 7660 of 14352 results