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pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain

Plasmid

#183387

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

redesigned Caspase-4 (AA94-377) (CASP4 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

ANR261ENP, D315H, M318K, T352K

Promoter

T7

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-mouse-Caspase-1-H340D

Plasmid

#183362

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1 H340D (Casp1 Mouse)

Use

Retroviral

Tags

Myc

Mutation

H340D

Promoter

MESV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-AKAR (mScarlet-I)

Plasmid

#181861

Purpose

FluoSTEP-AKAR using mScarlet-I as the FRET acceptor.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-AKAR (mScarlet-I)

Tags

mScarlet-I and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-58-DmeIF4Ga_687-1666_T

Plasmid

#147580

Purpose

Insect Expression of Dm4E-T_1-58-DmeIF4Ga_687-1666

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-58-DmeIF4Ga_687-1666 (4E-T Fly)

Expression

Insect

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-dog-Caspase-1/4a-triple mutant-H342D_K345M_K379T

Plasmid

#183364

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1/4a (CASP4 Canis lupus familiaris)

Use

Retroviral

Tags

Myc

Mutation

H342D_K345M_K379T

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-dog-Caspase-1/4b

Plasmid

#183365

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1/4b (CASP4 Canis lupus familiaris)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-dog-Caspase-1/4a

Plasmid

#183363

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1/4a (CASP4 Canis lupus familiaris)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetMP-CeSmg7_1-395-G80E_S

Plasmid

#147476

Purpose

Bacterial Expression of CeSmg7_1-395-G80E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg7_1-395-G80E (smg-7 Nematode)

Expression

Bacterial

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-510-V5His6_D

Plasmid

#146151

Purpose

Insect Expression of Dm4E-T_1-510

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-510 (4E-T Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T_1-510-V5His6_D

Plasmid

#146149

Purpose

Insect Expression of Dm4E-T_1-510

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-510 (4E-T Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDD2706

Plasmid

#175080

Purpose

Mammalian expression of Flag-tagged KSHV ORF45 RC mutant

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8)

Expression

Mammalian

Mutation

RC mutant; sequence mutation: GCGGCCGCAGCGCA

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDD2708

Plasmid

#175081

Purpose

Mammalian expression of Flag-tagged KSHV ORF45 RN1 mutant

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8)

Expression

Mammalian

Mutation

RN1 mutant: V284A/L285A

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDD2710

Plasmid

#175082

Purpose

Mammalian expression of Flag-tagged KSHV ORF45 RN2 mutant

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8)

Expression

Mammalian

Mutation

RN2 mutant: I289A/L291A

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDD2711

Plasmid

#175083

Purpose

Mammalian expression of Flag-tagged KSHV ORF45 carrying E223A-S226A/RC mutations

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8)

Expression

Mammalian

Mutation

E223A, S226A, RC mutations

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDD2712

Plasmid

#175084

Purpose

Mammalian expression of Flag tagged KSHV ORF45 carrying E223A-S226A/RN2 mutations

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8)

Expression

Mammalian

Mutation

E223A, S226A, RN2 mutations

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pTL337

Plasmid

#168398

Purpose

pGBKT7 yeast two-hybrid plasmid

Depositor

Sophien Kamoun

Article

Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

Insert

OJ1124_B05.17

Expression

Yeast

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Sc NybbR eRF1 (G180A)

Plasmid

#175415

Purpose

Encodes His-MBP-TEV-ybbR-G180A eRF1

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF1 (SUP45 Budding Yeast)

Tags

His-MBP-TEV and ybbR

Expression

Bacterial

Mutation

G180A

Promoter

T7

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNArodA

Plasmid

#149658

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBluescript-Q22YU3-KO

Plasmid

#170313

Purpose

Plasmid for generating Tetrahymena thermophila somatic knockout strains for Q22YU3 with Paromomycin selection

Depositor

Andrew Carter

Article

Mali et al Science. 2021 Feb 26;371(6532):910-916.

Insert

Q22YU3 knockout

Use

Tetrahymena somatic gene knockout vector

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBluescript-Q22MS1-KO

Plasmid

#170312

Purpose

Plasmid for generating Tetrahymena thermophila somatic knockout strains for Q22MS1 with Paromomycin selection

Depositor

Andrew Carter

Article

Mali et al Science. 2021 Feb 26;371(6532):910-916.

Insert

Q22MS1 knockout

Use

Tetrahymena somatic gene knockout vector

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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