We narrowed to 9,399 results for: sacs

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pAvA141

Plasmid

#248146

Purpose

mutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen2' mutations

Depositor

Emil Jensen

Article

van Aalst et al Commun Biol. 2025 Nov 27. doi: 10.1038/s

Insert

luxR (gen2 mutations)

Use

Integration vector

Tags

Gal4 activation domain and nuclear localization s…

Expression

Yeast

Mutation

Gal4_AD: G8V. NLS: K120N. LuxR: N86K, K104E, M135V

Promoter

pPGK1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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bRA66-Mic60chimeras

Plasmid

#245525

Purpose

Edited bRA66 containing gRNA to create S. cerevisiae Mic60 chimeras

Depositor

Luke Chao

Article

Benning et al Protein Sci. 2025 Jul;34(7):e70207. doi:

Insert

YKR016w (MIC60 Budding Yeast)

Use

CRISPR

Promoter

T7 TAATACGACTCACTATAGG

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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bRA66-deltaMic60

Plasmid

#245524

Purpose

Edited bRA66 containing gRNA to create S. cerevisiae deltaMic60 and S. cerevisiae Mic60(1-317)

Depositor

Luke Chao

Article

Benning et al Protein Sci. 2025 Jul;34(7):e70207. doi:

Insert

YKR016w (MIC60 Budding Yeast)

Use

CRISPR

Promoter

T7 TAATACGACTCACTATAGG

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-delta848-918

Plasmid

#238313

Purpose

ScVip1-536-1107-delta848-918 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-delta848-918

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-R547A-H548A-R551A

Plasmid

#238317

Purpose

ScVip1-536-1107-R547A-H548A-R551A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R547A-H548A-R551A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K554A-K556A-K644A

Plasmid

#238328

Purpose

ScVip1-536-1107-K554A-K556A-K644A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K554A-K556A-K644A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-P553V-G646V-G647A

Plasmid

#238322

Purpose

ScVip1-536-1107-P553V-G646V-G647A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-P553V-G646V-G647A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K817S-D819A-S821A

Plasmid

#238326

Purpose

ScVip1-536-1107-K817S-D819A-S821A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K817S-D819A-S821A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoED solvvol

Plasmid

#232958

Purpose

Galactose iduced expression of Gcn4 LVtoED solvvol in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED solvvol

Tags

TEV cleavage site

Expression

Yeast

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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PCDNA3.1:mTRESK

Plasmid

#224771

Purpose

In vitro transcription for Oocyte expression of mTRESK

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 18 (Kcnk18 Mouse)

Use

In vitro transcription for oocyte expression

Promoter

T7

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pETcon-pGAL1-Aga2-Htt(25Q)-c_myc

Plasmid

#225228

Purpose

Yeast optimized human Htt (25Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (25Q) in yeast surface display.

Depositor

Urartu Seker

Article

Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

Inserts

Aga2
Htt (25Q)

Tags

HA Tag and c-myc Tag

Expression

Yeast

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pETcon-pGAL1-Aga2-Htt(46Q)-c_myc

Plasmid

#225229

Purpose

Yeast optimized human Htt (46Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (46Q) in yeast surface display.

Depositor

Urartu Seker

Article

Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

Inserts

Aga2
Htt (46Q)

Tags

HA Tag and c-myc Tag

Expression

Yeast

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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ABEmax-Puro V2

Plasmid

#226955

Purpose

ABEmax plasmid enabling A:T to G:C base editing using a single plasmid.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAM90

Plasmid

#226682

Purpose

Rpn9S111TAG lid (Rpn5, MBP-HRV-Rpn6, Rpn8, Rpn9S111TAG, Rpn11)

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Inserts

Rpn5 (RPN5 Budding Yeast)
Rpn6 (RPN6 Budding Yeast)
Rpn8 (RPN8 Budding Yeast)
Rpn9 (RPN9 Budding Yeast)
Rpn11 (RPN11 Budding Yeast)

Tags

MBP-HRV

Expression

Bacterial

Mutation

S111TAG

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

Plasmid

#169135

Purpose

E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

Depositor

Michael Nash

Article

Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

Insert

Coh7

Tags

3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

Expression

Bacterial

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1:mTRESK(S116C)

Plasmid

#224772

Purpose

In vitro transcription for Oocyte expression of mTRESK(S116C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 18 (Kcnk18 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S116C

Promoter

T7

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(S118C/Q258K)

Plasmid

#224764

Purpose

In vitro transcription for Oocyte expression of hTRAAK(S118C/Q258K)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (KCNK4 Human)

Use

In vitro transcription for oocyte expression

Mutation

S118C/Q258K

Promoter

T7

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(S131C)

Plasmid

#224774

Purpose

Mammalian expression vector for generating mouse TREK-1

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Expression

Mammalian

Mutation

S131C

Promoter

CMV

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131C)

Plasmid

#224744

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (C159A/C219A)

Plasmid

#224751

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

C159A/C219A

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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