We narrowed to 15,232 results for: 110

1. lentiCRISPRv2_RB1#2

   Plasmid

   #174151

   Purpose

   Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   RB1 (RB1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. His-ZZ-TEV-SNAPf-DYNC2H1

   Plasmid

   #132524

   Purpose

   Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   DYNC2H1 (DYNC2H1 Human)
   

   Tags

   8x His, Linker-TEV-linker-TEV, Precission cleavag…

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0048-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q)

   Plasmid

   #103864

   Purpose

   dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR2DD (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   GSGGGGS and HIV NES

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-sgAMPKa2-Cas9-GFP

   Plasmid

   #208050

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa2

   Depositor

   Naama Kanarek

   Article

   Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

   Insert

   sgPRKAA2 (PRKAA2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223-NTRK3

   Plasmid

   #23901

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   NTRK3 (NTRK3 Human)
   

   Use

   Gateway donor vector

   Mutation

   F441L and L653P relative to NM_001012338.3

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188)

   Plasmid

   #159697

   Purpose

   E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188)

   Depositor

   Dominic Esposito

   Article

   Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

   Insert

   His6-MBP-tev-Hs.RAF1(52-188) (RAF1 Human)
   

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIG-702_CD19-BBz_CAR_tNGFR

   Plasmid

   #207485

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   tNGFR, CD19-BBz_CAR (NGFR Human)
   

   Use

   CRISPR

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIG-704_CD19-28z_CAR_tNGFR

   Plasmid

   #207486

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   tNGFR, CD19-28z_CAR (NGFR Human)
   

   Use

   CRISPR

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-SpyTag-Flag-SRSF1-V5-mCherry

   Plasmid

   #235089

   Purpose

   SRSF1 mCherry fusion protein without MCP domain

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   pCAG-SpyTag-Flag-SRSF1-V5-mCherry (SRSF1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET30-2-PHGDH

    Plasmid

    #83907

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    Phosphoglycerate dehydrogenase (PHGDH Human)
    

    Tags

    6x His tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1-kappa-myc-dL5-2xG4S-TMst

    Plasmid

    #73206

    Purpose

    Expresses myc-dL5(E52D)-TM (PDGFR derived) on the surface of mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

    Insert

    kappa-myc-dL5-2XG4S-TM (MYC Human)
    

    Tags

    The FAP is fused with 2 copies of a G4S linker an…

    Expression

    Mammalian

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    CMV

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-FLT4

    Plasmid

    #116745

    Purpose

    Lentiviral expression of FLT4

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FLT4 (FLT4 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223_IRF4_WT

    Plasmid

    #81782

    Purpose

    Gateway Donor vector containing IRF4 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    IRF4 (IRF4 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223-NTRK1

    Plasmid

    #23891

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    NTRK1 (NTRK1 Human)
    

    Use

    Gateway donor vector

    Mutation

    V263L relative to NM_001012331

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223-ROR2

    Plasmid

    #23927

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ROR2 (ROR2 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223-PIK3C2G

    Plasmid

    #23483

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PIK3C2G (PIK3C2G Human)
    

    Use

    Gateway donor vector

    Mutation

    C437T,3BP-del,1MM

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pEGFP-dnMCAK

    Plasmid

    #208650

    Purpose

    Mammalian expression plasmid expressing eGFP-dnMCAK driven by CMV promoter

    Depositor

    Samuel Bakhoum

    Article

    Li et al Nature. 2023 Aug;620(7976):1080-1088. do

    Insert

    Kif2c (Kif2c Cricetulus griseus (Chinese hamster))
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S92A , S106A , S108A, S112A , S186A, H530A, R534A…

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223-TRIB3

    Plasmid

    #23677

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    TRIB3 (TRIB3 Human)
    

    Use

    Gateway donor vector

    Mutation

    P5T

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pDONR223-PDK1

    Plasmid

    #23804

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PDK1 (PDK1 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130)

    Plasmid

    #145843

    Purpose

    Lentiviral vector genome to ectopically express human TMPRSS2 variant 1

    Depositor

    Caroline Goujon

    Article

    Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

    Insert

    human TMPRSS2 variant 1 (TMPRSS2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    G8V

    Promoter

    SFFV

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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