We narrowed to 15,991 results for: LEA

1. pGem-sHyper:sAPX

   Plasmid

   #84738

   Purpose

   Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Inserts

   sAPX (SAPX Mustard Weed)
   sHyper (HyPer2 targeted to nucleus)
   

   Tags

   nuclear localisation signals from the coding sequ…

   Expression

   Plant

   Promoter

   pFMV/p35S

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-Twin-Strep-TEV-DGKζ

   Plasmid

   #202531

   Purpose

   Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human diacylglycerol kinase ζ (DGKζ) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Insert

   DGKZ (DGKZ Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Promoter

   CAG and chicken β-actin promoter

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mNeonGreen-hCentrin1

   Plasmid

   #196890

   Purpose

   Neuron-specific expression of human (h)Centrin fused to mNeonGreen

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mNeonGreen-hCentrin1 (CETN1 Human)
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pelB-MBP-P13-ss

   Plasmid

   #137065

   Purpose

   E. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
   

   Tags

   PelB signal peptide + His10 + maltose binding pro…

   Expression

   Bacterial

   Mutation

   mature P13: lacks the P13 signal peptide

   Promoter

   T7lac

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGem-nHyPer:sAPX

   Plasmid

   #84735

   Purpose

   Simultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cells

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Inserts

   sAPX (SAPX Mustard Weed)
   nHyper (HyPer2 targeted to nucleus)
   

   Tags

   nuclear localisation signals from the coding sequ…

   Expression

   Plant

   Promoter

   FMV/35S

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3 ATP5O(5’UTR)-FF

   Plasmid

   #85486

   Purpose

   Firefly luciferase under the control of ATP5O 5'UTR

   Depositor

   Ivan Topisirovic

   Article

   Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

   Insert

   5'UTR of ATP5O (ATP5PO Human)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLU-CMV-Flag-hATG5 E122D

   Plasmid

   #111804

   Purpose

   Mammalian Expression of hATG5

   Depositor

   Jun Hee Lee

   Article

   Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

   Insert

   hATG5 (ATG5 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   E122D

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFPc1 hp47phox (S303/304/328D)

   Plasmid

   #112245

   Purpose

   expression of mutant p47phox

   Depositor

   Simone Di Giovanni

   Article

   Hervera et al Nat Cell Biol. 2018 Mar;20(3):307-319. d

   Insert

   p47phox (NCF1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S303/304/328D

   Promoter

   CMV

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSH1/M-FGFR3-Fv-Fvls-E

   Plasmid

   #15287

   Depositor

   David Spencer

   Article

   Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

   Insert

   FGFR3 kinase, FKBP12v36 (Fgfr3 Mouse)
   

   Tags

   Myristoylation-targeting domain c-Src (14aa) and …

   Expression

   Mammalian

   Mutation

   FGFR3 cytoplasmic domain only FKBP12 F36V

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUSEss-CHIg-mM-Asn209Asp_M18

    Plasmid

    #91781

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Asp (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Expression

    Mammalian

    Mutation

    Asn209 changed to Asp (EU numbering)

    Promoter

    hEF1-HTLV prom

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBJG1 T7-8xHis-OGT 5N5A

    Plasmid

    #154280

    Purpose

    Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

    Depositor

    Suzanne Walker

    Article

    Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Tags

    8x His, HRV3C protease site, and T7 leader

    Expression

    Bacterial

    Mutation

    N322A, N356A, N390A, N424A, N458A mutations; bact…

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-hPC1_4106-eGFP-3HA

    Plasmid

    #108399

    Purpose

    Express cytoplasmic tail human PC1 from amino acid 4106 to 4302

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-CD16.7-hPC1-eGFP-3HA

    Plasmid

    #108401

    Purpose

    Express cytoplasmic tail human PC1 from amino acid 4077 to 4302 with a membrane anchor

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE2-TetOminiCMV-SM

    Plasmid

    #136578

    Purpose

    Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2-E2A-cMyc (Sox2, Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBactin-Akna-mEGFP

    Plasmid

    #196866

    Purpose

    Expression of the centrosomal protein Akna fused to enhanced (E)-GFP

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Akna-mEGFP (Akna Mouse)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBactin-td-mCherry-Cdk5rap2

    Plasmid

    #196859

    Purpose

    Expression of Cyclin dependent kinase 5 regulatory subunit-associated protein 2 (Cdk5rap2) fused to tandem (td)-mCherry.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    td-mCherry-Cdk5rap2 (Cdk5rap2 Rat)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Control click editor - pCMV-T7-PCV2-nCas9-deadEcKlenow (JO520)

    Plasmid

    #208945

    Purpose

    A control CE1 construct with catalytically attenuated EcKlenow DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-dEcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A);deadEcKlenow(-exo;D355A,D357A,D705…

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. ISG15 C-term. Domain intein/chitin binding domain (79-156)

    Plasmid

    #110760

    Purpose

    Bacterial expression of ISG15 C-term.

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    ISG15 (ISG15 Human)
    

    Tags

    intein/chitin binding domain

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA4 h-Stx3 resistant to shRNA #304-2xMyc/His

    Plasmid

    #99746

    Purpose

    Expresses human Stx3 with a C-term myc-myc-his that is resistant to shRNA #304 from Sigma Aldrich.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

    Insert

    Stx3 (STX3 Human)
    

    Tags

    myc-myc-his

    Expression

    Mammalian

    Mutation

    Made six (6) silent mutations to confer resistanc…

    Promoter

    CMV/TO

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo

    Plasmid

    #112684

    Purpose

    An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    iRFP713
    

    Use

    AAV

    Tags

    2A-Flpo and Nuclear localization signal

    Promoter

    CMV-IE

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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