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WDR60

Plasmid

#132525

Purpose

Human WDR60, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

WDR60 (WDR60 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P04

Plasmid

#120015

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mTFP1

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mTFP1

Use

Pcr template

Tags

mTFP1

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSicoR-mCh-Chd1i4

Plasmid

#21908

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Insert

Chd1-i4

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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prNFL-P8R-EGFP

Plasmid

#132598

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P8R mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

23C>G; Pro8Arg

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-N98S-EGFP

Plasmid

#132600

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E N98S mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

293A>G; Asn98Ser

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-E397K-EGFP

Plasmid

#132603

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E E397K mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

1189G>A; Glu397Lys

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Desmocollin2_tail-GST

Plasmid

#36992

Depositor

Kathleen Green

Article

Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

Insert

Dsc2 (DSC2 Human)

Tags

GST

Expression

Bacterial

Mutation

Intracellular domain of Dsc2 (nt 2,603–3,158)

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only
S3_002_026_096

Plasmid

#134403

Purpose

pYTK096-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N

Tags

None and none

Expression

Yeast

Mutation

None and none

Promoter

pGPM1 and pRPL18B

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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TAL2314

Plasmid

#36740

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FANCF-TALEN_Left (Fancf Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only
P16ΔS:(MCS)-3xHA-S11

Plasmid

#108185

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

P16ΔS

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT L79E

Plasmid

#13640

Depositor

Luca Pellegrini

Article

Sik et al J Biol Chem. 2004 Apr 9. 279(15):15323-9

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Leu 79 to Glu

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
TAL2416

Plasmid

#36817

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FANCA-TALEN_Left (Fanca Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL-L1-1.3-DEL

Plasmid

#103035

Purpose

[Edit] Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by DEL domian

Depositor

Maria-Elena Torres-Padilla

Article

Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

Insert

TAL-L1-1.3-DEL

Use

T3 in vitro transcription for expression in mamma…

Tags

DEL and HA

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-3YFP-kanMX6

Plasmid

#105149

Purpose

Yeast gene targeting

Depositor

David Kovar, Thomas Pollard, Jian-Qiu Wu

Article

Wu et al J Cell Biol. 2006 Jul 31;174(3):391-402.

Insert

3YFP-kanMX6

Tags

3YFP

Expression

Bacterial and Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-Cα(K8,9A)-mPAGFP

Plasmid

#168493

Purpose

Mammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP

Expression

Mammalian

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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TAL2275

Plasmid

#36712

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MDM2-TALEN_Right (Mdm2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2400

Plasmid

#36803

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HDAC6-TALEN_Left (Hdac6 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2296

Plasmid

#36729

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BAX-TALEN_Left (Bax Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2297

Plasmid

#36730

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BAX-TALEN_Right (BAX Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
mCherry-SAC1C389S-FKBP

Plasmid

#108130

Purpose

Recruitable inactive SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:SACM1LC389S:FKBP(3-108) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(C389S)

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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