Addgene

We narrowed to 876 results for: GGCT;

Showing: 761 - 780 of 876 results

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Circular 200,100 (mIDUA)

Plasmid

#170123

Purpose

AAV vector carrying 2 copies of a guide RNA targeting the mouse IDUA mRNA, expressed from human and mouse U6 promoters

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA (Idua Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 and mouse U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with 8 bp bulge loops (GAPDH)

Plasmid

#170121

Purpose

AAV vector carrying a guide RNA targeting the human GAPDH mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with 8bp bulge loops (GAPDH Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSMPUW-miR-124-GFP-Puro

Plasmid

#117321

Purpose

Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

hsa-miR-124-1 (MIR124-1 Human)

Use

Lentiviral

Tags

GFP-Puro

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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PB09-TRE-beta-globin-miR-124-EF1a-GFP

Plasmid

#117318

Purpose

Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

hsa-miR-124-1 (MIR124-1 Human)

Use

Transposon-mediated integration

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Tet-inducible promoter

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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MAP4K4 gRNA (BRDN0001162353)

Plasmid

#76266

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP4K4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP4K4 (MAP4K4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-U6-sgMettl3

Plasmid

#196263

Purpose

Expresses a Mettl3-targeting sgRNA driven by the U6 promoter and Cre-recombinase driven by the PGK promoter

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

Mettl3 sgRNA (Mettl3 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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MAPK14 gRNA (BRDN0001148417)

Plasmid

#77924

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK14

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK14 (MAPK14 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK15 gRNA (BRDN0001145059)

Plasmid

#76483

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK15

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK15 (CDK15 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLCHKOv3-mCherry-intergenic-As

Plasmid

#209037

Purpose

Lentiviral vector expressing mCherry along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-SNCA-A53T

Plasmid

#181738

Purpose

Transiently expressing a pegRNA to introduce SNCA-A53T mutation in human cells

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for SNCA-A53T (SNCA Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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MAPK3 gRNA (BRDN0001149014)

Plasmid

#75966

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK3 (MAPK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MERTK gRNA (BRDN0001145505)

Plasmid

#76444

Purpose

3rd generation lentiviral gRNA plasmid targeting human MERTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MERTK (MERTK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-SNCA-A30P

Plasmid

#180016

Purpose

Transiently expressing a pegRNA to introduce SNCA-A30P mutation in human cells

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for SNCA-A30P (SNCA Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Tubb3gRNA-mCherry

Plasmid

#87111

Purpose

mouse Tubb3 target gRNA expression plasmid with CAGmCherry

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

Mouse Tubb3 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

CAG

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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EIF2AK1 gRNA (BRDN0001148541)

Plasmid

#77049

Purpose

3rd generation lentiviral gRNA plasmid targeting human EIF2AK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EIF2AK1 (EIF2AK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ALPK3 gRNA (BRDN0001146913)

Plasmid

#77325

Purpose

3rd generation lentiviral gRNA plasmid targeting human ALPK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ALPK3 (ALPK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ALPK1 gRNA (BRDN0001146035)

Plasmid

#76081

Purpose

3rd generation lentiviral gRNA plasmid targeting human ALPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ALPK1 (ALPK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CHEK2 gRNA (BRDN0001145610)

Plasmid

#76487

Purpose

3rd generation lentiviral gRNA plasmid targeting human CHEK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CHEK2 (CHEK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ALDH18A1 gRNA (BRDN0001145444)

Plasmid

#77995

Purpose

3rd generation lentiviral gRNA plasmid targeting human ALDH18A1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ALDH18A1 (ALDH18A1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AK2 gRNA (BRDN0001145434)

Plasmid

#76582

Purpose

3rd generation lentiviral gRNA plasmid targeting human AK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AK2 (AK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge