Efficient in vitro and in vivo RNA editing via recruitment of endogenous ADARs using circular guide RNAs.
Katrekar D, Yen J, Xiang Y, Saha A, Meluzzi D, Savva Y, Mali P
Nat Biotechnol. 2022 Feb 10. pii: 10.1038/s41587-021-01171-4. doi: 10.1038/s41587-021-01171-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170117 | Circular 100,50 (RAB7A) | AAV vector carrying a guide RNA targeting the human RAB7A mRNA |
| 170118 | Circular 200,100 (RAB7A) | AAV vector carrying a guide RNA targeting the human RAB7A mRNA |
| 170120 | Circular 200,100 (GAPDH) | AAV vector carrying a guide RNA targeting the human GAPDH mRNA |
| 170121 | Circular 200,100 with 8 bp bulge loops (GAPDH) | AAV vector carrying a guide RNA targeting the human GAPDH mRNA |
| 170122 | Circular 200,100 (mPCSK9) | AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters |
| 170123 | Circular 200,100 (mIDUA) | AAV vector carrying 2 copies of a guide RNA targeting the mouse IDUA mRNA, expressed from human and mouse U6 promoters |
| 180183 | Circular 200,100 with interspersed loops (GAPDH) | AAV vector carrying a guide RNA targeting the human GAPDH mRNA |
| 180184 | Circular guide cloning vector | Cloning vector for expressing circular RNA from a U6 promoter |
| 180191 | Circular 200,100 with interspersed loops (SMAD4) | AAV vector carrying a guide RNA targeting the human SMAD4 mRNA |
| 180192 | Circular 200,100 with interspersed loops (FANCC) | AAV vector carrying a guide RNA targeting the human FANCC mRNA |
| 180193 | Circular 200,100 with interspersed loops (CTNNB1) | AAV vector carrying a guide RNA targeting the human CTNNB1 mRNA |