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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,129 results for: gibson assembly

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Showing: 761 - 780 of 1129 results

  1. pBN095

    Plasmid
    #173765
    Purpose
    express E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    delete motif 3
    Promoter
    araBAD
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    173765-plasmid-map-sequence-id-344806

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  2. pBN215

    Plasmid
    #167962
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167962-plasmid-map-sequence-id-344789

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  3. pBN216

    Plasmid
    #167963
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y713E, Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167963-plasmid-map-sequence-id-344805

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  4. pBN217

    Plasmid
    #167964
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y708E, Y713E, Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167964-plasmid-map-sequence-id-344780

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  5. pBN261

    Plasmid
    #167966
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y713E, Y715E, Y717E
    Promoter
    araBAD
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167966-plasmid-map-sequence-id-344799

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  6. pBN262

    Plasmid
    #167967
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y708E, Y713E, Y715E, Y717E
    Promoter
    araBAD
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167967-plasmid-map-sequence-id-344775

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  7. pBN008

    Plasmid
    #167949
    Purpose
    express E. coli E69 (O9a:K30) Wzc with K540M mutation
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30), K540M nutant
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M
    Promoter
    araBAD
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167949-plasmid-map-sequence-id-344798

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  8. pBN202

    Plasmid
    #167950
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, Y713E, Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167950-plasmid-map-sequence-id-344771

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  9. pBN093

    Plasmid
    #167951
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, delete motif 3
    Promoter
    araBAD
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167951-plasmid-map-sequence-id-344776

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  10. pTEF1-UDH

    Plasmid
    #166679
    Purpose
    Yeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Insert
    UDH
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    TEF1
    Available Since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166679-plasmid-map-sequence-id-329863

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  16. pYZ033

    Plasmid
    #98404
    Purpose
    Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1
    Depositor
    Jef Boeke
    Article
    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Available Since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    98404-plasmid-map-sequence-id-198860

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  20. pETSUMO2_Vitiosangium-bGSDM-thrombin

    Plasmid
    #222099
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Vitiosangium-bGSDM-thrombin ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222099-plasmid-map-sequence-id-437094

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Showing: 761 - 780 of 1129 results