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We narrowed to 1,107 results for: gibson assembly

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Showing: 761 - 780 of 1107 results

  1. pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)

    Plasmid
    #181788
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181788-plasmid-map-sequence-id-354819

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  2. MZB184

    Plasmid
    #228488
    Purpose
    Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228488-plasmid-map-sequence-id-458447

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  3. C95m

    Plasmid
    #121001
    Purpose
    MoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    T7 RNA polymerase (Andy please check!)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121001-plasmid-map-sequence-id-236130

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  4. C77m

    Plasmid
    #120989
    Purpose
    MoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AmpR
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120989-plasmid-map-sequence-id-236056

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  5. C78m

    Plasmid
    #120990
    Purpose
    MoClo golden gate assembly CD cloning part for ChlorR (chloramphenicol resistance protein). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ChlorR
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120990-plasmid-map-sequence-id-236059

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  6. C32m

    Plasmid
    #120967
    Purpose
    MoClo golden gate assembly CD part for CinR (Quorum-sensing receptor for 3OC14). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohCD carb CinR
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120967-plasmid-map-sequence-id-236380

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  7. C36m

    Plasmid
    #120970
    Purpose
    MoClo golden gate assembly CD part for RhlI (quorum-sensing synthase for 3OC14). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohCD carb RhlI
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120970-plasmid-map-sequence-id-245423

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  8. C40m

    Plasmid
    #120973
    Purpose
    MoClo golden gate assembly CD part for blank part (random 50bp sequence) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Blank (Random 50bp sequence)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120973-plasmid-map-sequence-id-236027

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  9. C20m

    Plasmid
    #120961
    Purpose
    MoClo golden gate assembly CD part with sfYFP-ssrA (superfolder YFP with ssrA degradation tag; variant of https://www.fpbase.org/protein/yfp/)
    Depositor
    Richard Murray
    Insert
    sfYFP-ssrA (has BbsI site)
    Use
    Synthetic Biology
    Tags
    ssrA degradation tag
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120961-plasmid-map-sequence-id-235979

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  12. MZB223

    Plasmid
    #228491
    Purpose
    Empty C-terminal 8xHis tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    8xHis
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228491-plasmid-map-sequence-id-455392

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  13. MZB116

    Plasmid
    #228480
    Purpose
    "Empty" C-terminal self-cleaving CPD-tag vector for rapid cloning/DNA Assembly via SwaI restriction enzyme site (bacterial expression)
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    CPD-10xHis
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    228480-plasmid-map-sequence-id-455378

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  14. MZB133

    Plasmid
    #228484
    Purpose
    "Empty" N-terminal TEV cleavable 6x His tag vector for rapid cloning/DNA Assembly via SmaI restriction site (Bacterial Expression)
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228484-plasmid-map-sequence-id-457332

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  15. V35m

    Plasmid
    #121042
    Purpose
    MoClo golden gate assembly AE destination vector DVCK-AE-GFP (ColE1/kan resistance destination vector with constitutive GFP expression for screening.
    Depositor
    Richard Murray
    Insert
    Kan destination vector with ColE1 origin and GFP selection
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121042-plasmid-map-sequence-id-247704

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  16. T1m

    Plasmid
    #121028
    Purpose
    MoClo golden gate assembly DE part for T500 terminator Engineered terminator derived from bacteriophage 83; see DOI: 10.1126/science.284.5414.611).
    Depositor
    Richard Murray
    Insert
    T500_noGap, short attachment (T11)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121028-plasmid-map-sequence-id-245901

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Showing: 761 - 780 of 1107 results