We narrowed to 1,068 results for: gibson assembly

1. pAAV-CAG-SuperNovaGreen-T2A-dTomato

   Plasmid

   #193798

   Purpose

   Expresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   SuperNova Green
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPW3760 : CMVd3-HsIRE1-K599A_KinaseDead-HaloTag

   Plasmid

   #185682

   Purpose

   Low-level transient expression of kinase-dead K599A HsIRE1 with a C-terminal HaloTag.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERN1 (ERN1 Human, Synthetic)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   K599A

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TOPO tTR-KRAB-rtTA3-Luc

   Plasmid

   #68449

   Purpose

   TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   tTR-KRAB-2A-rtTA3-2A-Luciferase
   

   Use

   Luciferase; Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPW3759 : CMVd3-HsIRE1deltaLD-HaloTag

   Plasmid

   #185681

   Purpose

   Low-level transient expression of delta-lumenal domain HsIRE1 with a C-terminal HaloTag.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERN1 (ERN1 Human, Synthetic)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Deletion of lumenal domain

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPW3762 : CMVd3-HsIRE1(K121Y)-HaloTag

   Plasmid

   #185684

   Purpose

   Low-level transient expression of IF1 mutant HsIRE1 with a C-terminal HaloTag.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERN1 (ERN1 Human, Synthetic)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   K121Y

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-CAG-miniSOG-T2A-dTomato

   Plasmid

   #193796

   Purpose

   Expresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   miniSOG
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Integrin β6β1-EGFP

   Plasmid

   #242829

   Purpose

   Encodes human integrin β6 chimera containing integrin β1 cytoplasmic domain and C-terminal EGFP tag

   Depositor

   Cheng-han Yu

   Article

   Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

   Insert

   Integrin subunit beta 6, transcript variant 1 (ITGB6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Integrin β6 chimera containing the cytoplasmic ta…

   Promoter

   Available since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHS1625

   Plasmid

   #239834

   Purpose

   OrufIscB-REC E. coli expression for recombinant purification

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

   Insert

   OrufIscB-REC
   

   Use

   Tags

   14xHis-TwinStrep-bdSUMO

   Expression

   Bacterial

   Mutation

   Insertion of REC domain from NbaCas9-1

   Promoter

   T7

   Available since

   July 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHS1505

   Plasmid

   #239830

   Purpose

   OrufIscB-REC mammalian expression

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

   Insert

   OrufIscB-REC
   

   Use

   Tags

   Nucleoplasmin NLS-3xHA and SV40 NLS

   Expression

   Mammalian

   Mutation

   Insertion of REC domain from NbaCas9-1

   Promoter

   CMV

   Available since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETSUMO2_Ideonella-bGSDM-WELQ

    Plasmid

    #222098

    Purpose

    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Ideonella species with a WELQut protease cleavage site for controlled activation.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

    Insert

    Ideonella-bGSDM-WELQ ORF
    

    Use

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pL0_BC-pyr_comp_sig_pep

    Plasmid

    #202132

    Purpose

    Level 0 plasmid containing a kozak consensus sequence and a pyrenoid compartment signal peptide sequence with BC overhangs used to build level 1 constructs.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    Pyrenoid compartment signal peptide
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Sept. 6, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pL0-EF-54730

    Plasmid

    #202163

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 54730 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    54730 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Sept. 6, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pL0_AC-27877

    Plasmid

    #202110

    Purpose

    Level 0 plasmid containing the promoter from gene 27877 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    27877 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove sapI sites fo…

    Promoter

    Available since

    Aug. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pL0_CE-OriT

    Plasmid

    #202150

    Purpose

    Level 0 plasmid containing an origin of transfer sequence from pPtPBR11 required for making plasmids mobilizable for conjugation with CE overhangs used to build level 1 constructs.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    Origin of transfer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    OriT sequence mutated to remove sapI sites domest…

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pL0_AC-Fld

    Plasmid

    #202125

    Purpose

    Level 0 plasmid containing the promoter from gene 23658 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    Fld promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove bsaI site for…

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pL0_AC-54730

    Plasmid

    #202109

    Purpose

    Level 0 plasmid containing the promoter from gene 54730 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    54730 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove bsaI site for…

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pL0_AC-51183

    Plasmid

    #202108

    Purpose

    Level 0 plasmid containing the promoter from gene 51183 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    51183 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove sapI sites fo…

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pL0_AC-GEF

    Plasmid

    #202107

    Purpose

    Level 0 plasmid containing the promoter from gene 41365 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    GEF promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove sapI site for…

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pL0_AC-47740

    Plasmid

    #202106

    Purpose

    Level 0 plasmid containing the promoter from gene 47740 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    47740 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove bsaI site for…

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pL0_AC-BCA

    Plasmid

    #202105

    Purpose

    Level 0 plasmid containing the promoter from gene 51305 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    BCA promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove sapI sites fo…

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge