We narrowed to 1,124 results for: gibson assembly

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pL0_AC-act1

Plasmid

#202113

Purpose

Level 0 plasmid containing the promoter from gene 29812 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

act1 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-CaMK2

Plasmid

#202112

Purpose

Level 0 plasmid containing the promoter from gene 39236 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

CaMK2 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-fbaC5

Plasmid

#202111

Purpose

Level 0 plasmid containing the promoter from gene 41423 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

fbaC5 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-OEE3

Plasmid

#202103

Purpose

Level 0 plasmid containing the promoter from gene 54499 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

OEE3 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-P49

Plasmid

#202102

Purpose

Level 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

P49 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-NR

Plasmid

#202101

Purpose

Level 0 plasmid containing the promoter from gene 54983 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

NR promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-H4

Plasmid

#202100

Purpose

Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

H4 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pBN095

Plasmid

#173765

Purpose

express E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

delete motif 3

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN215

Plasmid

#167962

Purpose

express E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y715E, Y717E, Y718E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN216

Plasmid

#167963

Purpose

express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y713E, Y715E, Y717E, Y718E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN217

Plasmid

#167964

Purpose

express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y708E, Y713E, Y715E, Y717E, Y718E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN261

Plasmid

#167966

Purpose

express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y713E, Y715E, Y717E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN262

Plasmid

#167967

Purpose

express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y708E, Y713E, Y715E, Y717E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN008

Plasmid

#167949

Purpose

express E. coli E69 (O9a:K30) Wzc with K540M mutation

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30), K540M nutant

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN202

Plasmid

#167950

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y713E, Y715E, Y717E, Y718E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN093

Plasmid

#167951

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, delete motif 3

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-MIOX only

Plasmid

#166683

Purpose

Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Insert

GFP-MIOX

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL10

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTEF1-UDH

Plasmid

#166679

Purpose

Yeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Insert

UDH

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF1

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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wtVP1 only

Plasmid

#166673

Purpose

Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Insert

Wild-type Murine polyomavirus VP1

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL1

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 Q147A, M151A, I154A

Plasmid

#74616

Purpose

Expresses a triple mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Q147A, M151A, I154A

Promoter

T7

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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