We narrowed to 1,029 results for: gibson assembly

1. pPW3762 : CMVd3-HsIRE1(K121Y)-HaloTag

   Plasmid

   #185684

   Purpose

   Low-level transient expression of IF1 mutant HsIRE1 with a C-terminal HaloTag.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERN1 (ERN1 Human, Synthetic)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   K121Y

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-CAG-miniSOG-T2A-dTomato

   Plasmid

   #193796

   Purpose

   Expresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   miniSOG
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPW3763 : CMVd3-HsIRE1dLKR-HaloTag

   Plasmid

   #185685

   Purpose

   Low-level transient expression of HsIRE1 lacking the linker, kinase, and RNase domains, with a C-terminal HaloTag.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERN1 (ERN1 Human, Synthetic)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Deletion of linker, kinase, and Rnase domains

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pETSUMO2_Ideonella-bGSDM-WELQ

   Plasmid

   #222098

   Purpose

   For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Ideonella species with a WELQut protease cleavage site for controlled activation.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

   Insert

   Ideonella-bGSDM-WELQ ORF
   

   Use

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETSUMO2_Vitiosangium-bGSDM-thrombin

   Plasmid

   #222099

   Purpose

   For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

   Insert

   Vitiosangium-bGSDM-thrombin ORF
   

   Use

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETSUMO2_Nostoc-bGSDM-WELQ

   Plasmid

   #222100

   Purpose

   For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

   Insert

   Nostoc-bGSDM-WELQ ORF
   

   Use

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pL0_BC-pyr_comp_sig_pep

   Plasmid

   #202132

   Purpose

   Level 0 plasmid containing a kozak consensus sequence and a pyrenoid compartment signal peptide sequence with BC overhangs used to build level 1 constructs.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   Pyrenoid compartment signal peptide
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Sept. 6, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pL0-EF-54730

   Plasmid

   #202163

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 54730 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   54730 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Sept. 6, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pL0_AC-27877

   Plasmid

   #202110

   Purpose

   Level 0 plasmid containing the promoter from gene 27877 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   27877 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter sequence mutated to remove sapI sites fo…

   Promoter

   Available since

   Aug. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pL0_CE-OriT

    Plasmid

    #202150

    Purpose

    Level 0 plasmid containing an origin of transfer sequence from pPtPBR11 required for making plasmids mobilizable for conjugation with CE overhangs used to build level 1 constructs.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    Origin of transfer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    OriT sequence mutated to remove sapI sites domest…

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pL0_AC-Fld

    Plasmid

    #202125

    Purpose

    Level 0 plasmid containing the promoter from gene 23658 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    Fld promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove bsaI site for…

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pL0_AC-54730

    Plasmid

    #202109

    Purpose

    Level 0 plasmid containing the promoter from gene 54730 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    54730 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove bsaI site for…

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pL0_AC-51183

    Plasmid

    #202108

    Purpose

    Level 0 plasmid containing the promoter from gene 51183 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    51183 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove sapI sites fo…

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pL0_AC-GEF

    Plasmid

    #202107

    Purpose

    Level 0 plasmid containing the promoter from gene 41365 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    GEF promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove sapI site for…

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pL0_AC-47740

    Plasmid

    #202106

    Purpose

    Level 0 plasmid containing the promoter from gene 47740 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    47740 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove bsaI site for…

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pL0_AC-BCA

    Plasmid

    #202105

    Purpose

    Level 0 plasmid containing the promoter from gene 51305 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    BCA promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove sapI sites fo…

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pL0_AC-epyC

    Plasmid

    #202104

    Purpose

    Level 0 plasmid containing the promoter from gene 41316 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    epyC promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove bsaI sites fo…

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pAAV-CAG-miniSOG-C-Far

    Plasmid

    #193914

    Purpose

    Control plasmid that expresses miniSOG targeted to the cell membrane in mammalian cells

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Farnesylation signal sequence and Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. VP2C-GFP-MIOX only

    Plasmid

    #166684

    Purpose

    Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Insert

    VP2C-GFP-MIOX
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. deltaVP1 only

    Plasmid

    #166675

    Purpose

    Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Insert

    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge