We narrowed to 7,650 results for: ski

1. pCellFree_G03 WWP1

   Plasmid

   #67125

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   WWP1 (WWP1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSETT4gap

   Plasmid

   #153413

   Purpose

   φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette

   Depositor

   Joern Kalinowski

   Article

   Schaffert et al Microbiol Resour Announc. 2020 Sep 24;9(

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   gapDH promoter from Eggerthella lenta

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDNA3-AKAP1-mCherry-LOVPLD

   Plasmid

   #219558

   Purpose

   mitochondria-targeted LOVPLD

   Depositor

   Jeremy Baskin

   Article

   Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

   Insert

   AKAP1-mCherry-LOVPLD
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDNA3-lyn10-mCherry- LOVPLD-P (LOVPLD)

   Plasmid

   #219555

   Purpose

   PM-targeted LOVPLD

   Depositor

   Jeremy Baskin

   Article

   Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

   Insert

   lyn10-mCherry-LOVPLD
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCellFree_G04

   Plasmid

   #67138

   Purpose

   Gateway destination vector for cell-free expression with C-term eGFP 8xHis tag

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

   Type

   Empty backbone

   Use

   Cell-free expression

   Tags

   EGFP and HIS

   Available Since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pICSL50005

   Plasmid

   #117536

   Purpose

   Level0 Golden Gate part: c-terminal tag, YFP

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   c-term tag YFP
   

   Use

   Synthetic Biology

   Mutation

   G65S, L68V, A72S, Y203T, L231H

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCellFree_G02

   Plasmid

   #67136

   Purpose

   Gateway destination vector for cell-free expression with C-term 8xHis tag

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

   Type

   Empty backbone

   Use

   Cell-free expression

   Tags

   His

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCellFree_G03 POLD1

   Plasmid

   #67128

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   POLD1 (POLD1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)

   Plasmid

   #173453

   Purpose

   Contains mCherry-mNeonGreen tandem fluorescent protein timer

   Depositor

   Anton Khmelinskii

   Article

   Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

   Insert

   mCherry-mNeonGreen
   

   Expression

   Yeast

   Promoter

   no promoter

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Endosome-targeted optodPLD

    Plasmid

    #140055

    Purpose

    Encodes CRY2-mCherry-dPLD-P2A-CIBN-2xFYVE for mammalian expression

    Depositor

    Jeremy Baskin

    Article

    Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Phospholipase D
    

    Tags

    2xFYVE, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    H170A (catalytic dead)

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pU6-TREX1 g1 Cas9-T2A-mCherry

    Plasmid

    #164250

    Purpose

    Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgTREX1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCellFree_G03 TFAP2B

    Plasmid

    #67092

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TFAP2B (TFAP2B Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEPOR0SP0020

    Plasmid

    #117531

    Purpose

    Level0 Golden Gate part: CDS, SaCas9

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SaCas9 (with stop codon)
    

    Use

    CRISPR

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCellFree_G03 RAD54B

    Plasmid

    #67124

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    RAD54B (RAD54B Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pJEP230-Lenti-TRE3G-4xMyc-GluN2B(E1479Q)-WPRE-pA

    Plasmid

    #164795

    Purpose

    pLenti vector backbone designed to express 4XMyc Tagged-GluN2B(E1479Q) from a TRE3G promoter.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    GluN2B
    

    Use

    Lentiviral

    Tags

    4xMyc

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pC-PTP-NEO

    Plasmid

    #51710

    Purpose

    contains a C-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

    Depositor

    Arthur Gunzl

    Article

    Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

    Type

    Empty backbone

    Use

    T. brucei genome integration vectors

    Tags

    PTP (ProtC-TEV-ProtA)

    Available Since

    March 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIRES_iSeroSnFR_cytosolic

    Plasmid

    #214132

    Purpose

    Fluorescent biosensor for intracellular serotonin (5-HT)

    Depositor

    Steffen Sinning

    Article

    Bukowski et al Research Square PREPRINT. 18 August 2023

    Insert

    iSeroSnFR_cytosolic
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCHNFI-B2

    Plasmid

    #31405

    Depositor

    Richard Gronostajski

    Article

    Chaudhry et al J Biol Chem. 1998 Jul 17. 273(29):18538-

    Insert

    Nuclear Factor IB isoform 2 (Nfib Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Not the same as reference seq. See sequence for m…

    Available Since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pN-PURO-PTP

    Plasmid

    #51711

    Purpose

    contains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

    Depositor

    Arthur Gunzl

    Article

    Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

    Type

    Empty backbone

    Use

    T. brucei genome integration vectors

    Tags

    PTP (ProtC-TEV-ProtA)

    Available Since

    March 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMLC-BB1

    Plasmid

    #166922

    Purpose

    Backbone for assembly of multi-level gene expression controllers

    Depositor

    Thomas Gorochowski

    Article

    Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

    Insert

    CyOFP - Orange fluorescent protein - flanked by BsaI sites
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23100 constitutive

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge