We narrowed to 7,701 results for: ski

1. pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)

   Plasmid

   #173456

   Purpose

   Contains mScarletH-mNeonGreen tandem fluorescent protein timer

   Depositor

   Anton Khmelinskii

   Article

   Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

   Insert

   mScarlet-H-mNeonGreen
   

   Expression

   Yeast

   Promoter

   no promoter

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Golgi-targeted optodPLD

   Plasmid

   #140057

   Purpose

   Encodes CRY2-mCherry-dPLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expression

   Depositor

   Jeremy Baskin

   Article

   Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Phospholipase D
   

   Tags

   CIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…

   Expression

   Mammalian

   Mutation

   H170A (catalytic dead)

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCellFree_G03 WWP1

   Plasmid

   #67125

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   WWP1 (WWP1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-TREX1 g2 Cas9-T2A-mCherry

   Plasmid

   #164251

   Purpose

   Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   sgTREX1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCellFree_G03 CYP2A13

   Plasmid

   #67103

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CYP2A13 (CYP2A13 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pJEP228-Lenti-CMV-GFP-GluN2B-WPRE-pA

   Plasmid

   #84277

   Purpose

   pLenti vector backbone designed to express GFP tagged GluN2B from a CMV promoter.

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   GluN2B
   

   Use

   Lentiviral

   Tags

   GFP

   Promoter

   CMV

   Available Since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJEP227-Lenti-CMV-GFP-GluN2A-WPRE-pA

   Plasmid

   #84276

   Purpose

   pLenti vector backbone designed to express GFP tagged GluN2A from a CMV promoter.

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   GluN2A
   

   Use

   Lentiviral

   Tags

   GFP

   Promoter

   CMV

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Vh-HA-3xFRB

   Plasmid

   #20223

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   3xFRB (MTOR Human)
   

   Tags

   HA and Vh

   Expression

   Mammalian

   Mutation

   One copy of FRB has a T2098L mutation

   Available Since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pSETT4gap

   Plasmid

   #153413

   Purpose

   φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette

   Depositor

   Joern Kalinowski

   Article

   Schaffert et al Microbiol Resour Announc. 2020 Sep 24;9(

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   gapDH promoter from Eggerthella lenta

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMC-J6-dCas9-24XGP41-J9

    Plasmid

    #202006

    Purpose

    MoClo level 0 dCas9-24XGP41 coding sequence module

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

    Insert

    dCas9-24XGP41
    

    Use

    Synthetic Biology; Moclo cloning vector

    Tags

    GS linkers

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCellFree_G03 POLD1

    Plasmid

    #67128

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    POLD1 (POLD1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCDNA3-AKAP1-mCherry-LOVPLD

    Plasmid

    #219558

    Purpose

    mitochondria-targeted LOVPLD

    Depositor

    Jeremy Baskin

    Article

    Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

    Insert

    AKAP1-mCherry-LOVPLD
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCellFree_G02

    Plasmid

    #67136

    Purpose

    Gateway destination vector for cell-free expression with C-term 8xHis tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    His

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCellFree_G04

    Plasmid

    #67138

    Purpose

    Gateway destination vector for cell-free expression with C-term eGFP 8xHis tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    EGFP and HIS

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICSL50005

    Plasmid

    #117536

    Purpose

    Level0 Golden Gate part: c-terminal tag, YFP

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    c-term tag YFP
    

    Use

    Synthetic Biology

    Mutation

    G65S, L68V, A72S, Y203T, L231H

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Endosome-targeted optodPLD

    Plasmid

    #140055

    Purpose

    Encodes CRY2-mCherry-dPLD-P2A-CIBN-2xFYVE for mammalian expression

    Depositor

    Jeremy Baskin

    Article

    Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Phospholipase D
    

    Tags

    2xFYVE, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    H170A (catalytic dead)

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)

    Plasmid

    #173453

    Purpose

    Contains mCherry-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-mNeonGreen
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCellFree_G03 TFAP2B

    Plasmid

    #67092

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TFAP2B (TFAP2B Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pU6-TREX1 g1 Cas9-T2A-mCherry

    Plasmid

    #164250

    Purpose

    Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgTREX1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCellFree_G03 RAD54B

    Plasmid

    #67124

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    RAD54B (RAD54B Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only