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pCMV-ABEmax-NRTH

Plasmid

#136922

Purpose

Expresses ABEmax-NRTH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

ABEmax-NRTH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-V2006I

Plasmid

#69012

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

V2006I

Promoter

CMV

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry-sesRNA-GFP-WPRE-pA

Plasmid

#192065

Purpose

Expression of mCherry, sesRNA in mammalian cells, with GFP as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for Cheta

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ14806

Plasmid

#239273

Purpose

Expresses TRF1-PYL1 for recruiting RNA to the nuclear stress body via CRISPR-TO

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

HSF1-BFP-PYL1 (HSF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_empty

Plasmid

#160093

Purpose

Control lentiviral vector for mammalian gene expression

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE

Plasmid

#196678

Purpose

AAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.

Depositor

Haining Zhong

Article

Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

Insert

PKIαmut-P2A-mRuby3

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV-NLS-ISDge10-NLS-3xHA-bGHpA;U6-BsaI-reRNA

Plasmid

#205318

Purpose

All-in-one AAV vector expressing ISDge10 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

humanized ISDge10 TnpB

Use

AAV

Tags

3 x HA

Expression

Mammalian

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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cycLuc_TEVS

Plasmid

#119207

Purpose

Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

cyclic luciferase with TEVs cleavage site

Use

Luciferase

Tags

6xHis and AU1 tag

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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NO MS-Sensor (35)

Plasmid

#199783

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

NO MS-Sensor

Use

Synthetic Biology

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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nLuc-PTHR1

Plasmid

#162577

Purpose

Expresses N-terminally NanoLuc-Tagged PTHR1 in mammalian cells

Depositor

Sam Gellman

Article

Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3

Insert

Parathyroid Hormone Receptor 1 (PTH1R Human)

Tags

Nanoluc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax-NRCH

Plasmid

#136923

Purpose

Expresses ABEmax-NRCH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

ABEmax-NRCH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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gCH27 (crCD55-4)

Plasmid

#217343

Purpose

crRNA targeting CD55

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

crCD55-4 (CD55 Human)

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloAKAR2.1-CLC

Plasmid

#216492

Purpose

Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) targeted to clathrin coated pits

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.1-CLC

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-TREG3G-PE2-rtTA3G-P2A-eGFP

Plasmid

#196971

Purpose

Piggybac vector with doxycyclin-inducible prime editor for mammalian cells

Depositor

Leopold Parts

Article

Koeppel et al Nat Biotechnol. 2023 Feb 16. doi: 10.103

Inserts

Cas9-RT
rtTA3G-P2A-eGFP

Expression

Mammalian

Promoter

PGK and TREG3G

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA-GFP

Plasmid

#200068

Purpose

For the knock down of GFP with an astrocyte specific mCherry expression

Depositor

Baljit Khakh

Article

Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

Insert

U6-sgRNA-GFP

Use

AAV, CRISPR, and Mouse Targeting

Tags

GfaABC1D-mCherry

Promoter

U6, GfaABC1D

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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xCas9

Plasmid

#138565

Purpose

Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

xCas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-sL7-Cre-HA-WPRE

Plasmid

#204488

Purpose

For production of driver AAV virus for Purkinje cell-specific expression

Depositor

Samuel Wang

Article

Chen et al Nature. 2022 May;605(7911):722-727. doi:

Insert

Cre

Use

AAV, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Promoter

sL7

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pKH115_GALNT2-keima

Plasmid

#214969

Purpose

Golgiphagy flux reporter GALNT2-keima

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

GALNT2-keima (GALNT2 Human)

Use

Lentiviral

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgNT

Plasmid

#138681

Purpose

Expresses a non-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNT

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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B2345_BTB2x-mCherry

Plasmid

#215097

Purpose

Expresses BCL6 BTB(1-129)-BTB(1-129)-mCherry in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BCL6 BTB-BTB (BCL6 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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