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MADR pDonor TagBFP2-3XFlag (cyto) WPRE

Plasmid

#129421

Purpose

MADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter protein

Depositor

Joshua Breunig

Article

Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

Insert

TagBFP2-3Flag

Use

Mosaic analysis for dual recombinase-mediated cas…

Tags

3X Flag epitope tag

Promoter

none (3X polyA to mitigate episomal expression)

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-NEK3

Plasmid

#23821

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK3 (NEK3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223_EGFR_p.ELREA746del_T790M

Plasmid

#82917

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

T790M; p.ELREA746del

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-SRPK3

Plasmid

#23440

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SRPK3 (SRPK3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223_LAP3_WT_V5

Plasmid

#82958

Purpose

Gateway Donor vector containing LAP3, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

LAP3 (LAP3 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-RAGE

Plasmid

#23645

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RAGE (MOK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-STK32B

Plasmid

#23795

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK32B (STK32B Human)

Use

Gateway donor vector

Mutation

H5L

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-STK32A

Plasmid

#23770

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK32A (STK32A Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-SCYL3

Plasmid

#23680

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SCYL3 (SCYL3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223_GABPB1_WT_V5

Plasmid

#82972

Purpose

Gateway Donor vector containing GABPB1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

GABPB1 (GABPB1 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-PAPSS1

Plasmid

#23868

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PAPSS1 (PAPSS1 Human)

Use

Gateway donor vector

Mutation

2MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-STK25

Plasmid

#23800

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK25 (STK25 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-YSK4

Plasmid

#23616

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

YSK4 (YSK4 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
MSCV-Neo-GFP/V5-Foxa1-(P2A)-Flag-Gata5

Plasmid

#105509

Purpose

MSCV-driven retroviral Foxa1 and Gata5 expression (P2A-linked, V5 and Flag-tagged each)

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Inserts

Foxa1 (Foxa1 Mouse)
Gata5 (Gata5 Mouse)

Use

Retroviral

Tags

Flag and V5

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_DCAF8_WT_V5

Plasmid

#82947

Purpose

Gateway Donor vector containing DCAF8, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

DCAF8 (DCAF8 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-TPR

Plasmid

#23519

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TPR (TPR Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PDXK

Plasmid

#23780

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDXK (PDXK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PLXNB2

Plasmid

#23407

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PLXNB2 (PLXNB2 Human)

Use

Gateway donor vector

Mutation

Q167R

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223_DARC_WT_V5

Plasmid

#82973

Purpose

Gateway Donor vector containing DARC, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ACKR1 (ACKR1 Human)

Use

Gateway entry vector

Mutation

F326S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKACG

Plasmid

#23788

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKACG (PRKACG Human)

Use

Gateway donor vector

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only