We narrowed to 18,501 results for: MUT

1. GFP-cCGNL1-Myc

   Plasmid

   #236516

   Purpose

   For expression of Paracingulin (dog) in mammalian cells

   Depositor

   Sandra Citi

   Article

   Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

   Insert

   CGNL1 (CGNL1 Canis lupus familiaris (dog))
   

   Tags

   GFP and Myc

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. A1-LCD allW

   Plasmid

   #234609

   Purpose

   Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.

   Depositor

   Tanja Mittag

   Article

   Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

   Insert

   hnRNPA1_LCD_allW (HNRNPA1 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   C-terminal domain (186-320) with all aromatic ami…

   Promoter

   T7

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pScalps-Puro-FLAG_HA-Regnase-1 D141N

   Plasmid

   #222663

   Purpose

   Lentiviral vector to express mouse Regnase-1 D141N mutant tagged with FLAG-HA at N-term

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   Zc3h12a (Zc3h12a Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   mutated aspartic acid 141 to asparagine to abroga…

   Promoter

   SFFV

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-MARK2-K82H-CR

   Plasmid

   #229425

   Purpose

   Lentiviral or overpexression of cDNA

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   MARK2-K82H (MARK2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Kinase-dead mutant K82H; silent mutation CRISPR r…

   Promoter

   EFS

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-MARK3-GK-CR

   Plasmid

   #229424

   Purpose

   Lentiviral or overpexression of cDNA

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   MARK3-GK-CR (MARK3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Gatekeeper mutation M132G; silent mutation CRISPR…

   Promoter

   EFS

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-MARK2-GK-CR

   Plasmid

   #229423

   Purpose

   Lentiviral or overpexression of cDNA

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   MARK2-GK-CR (MARK2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Gatekeeper mutation M129G; silent mutation CRISPR…

   Promoter

   EFS

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cdk13_intron3_sg3_pX458

   Plasmid

   #127338

   Purpose

   sgRNA that cuts within intron 3 of mouse CDK13 genomic locus- guide #3

   Depositor

   Phil Sharp

   Article

   Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

   Insert

   Mouse Cdk13 Intron 3 sgRNA
   

   Use

   CRISPR and Mouse Targeting

   Promoter

   U6 Promoter

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cdk13_intron4_sg4_pX330

   Plasmid

   #127341

   Purpose

   sgRNA that cuts within intron 4 of mouse CDK13 genomic locus- guide #4

   Depositor

   Phil Sharp

   Article

   Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

   Insert

   Mouse Cdk13 Intron 4 Targeting sgRNA
   

   Use

   CRISPR and Mouse Targeting

   Promoter

   U6 Promoter

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a(+)-NEDD4L_W3-W4-HECT_C874only

   Plasmid

   #187711

   Purpose

   Express his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 492-975 with mutations (NEDD4L Human)
   

   Tags

   His-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-491; mutations C702A, C776…

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pScalps-Puro-FLAG-HA-Regnase-3 D252N

    Plasmid

    #222664

    Purpose

    Lentiviral vector to express mouse Regnase-3 D252N mutant tagged with FLAG-HA at N-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c (Zc3h12c Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    mutated aspartic acid 252 to asparagine to abroga…

    Promoter

    SFFV

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFB1.HMBP.PrS.CBX8-KR21A

    Plasmid

    #224709

    Purpose

    Expresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 KR21A mt (CBX8 Human)
    

    Tags

    HMBP

    Expression

    Insect

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. CIRC#del

    Plasmid

    #186344

    Purpose

    Contains VEGFA exon 7 and short intronic flanking regions not sufficient for expression of hsa_circ_0076611 (circVEGF)

    Depositor

    Giovanni Blandino

    Article

    Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

    Insert

    VEGFA (VEGFA Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcs2-cDELE1(142-515)-sumo

    Plasmid

    #226107

    Purpose

    cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assays

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    DELE1 (DELE1 Human)
    

    Tags

    Sumo

    Expression

    Mammalian

    Mutation

    encodes only residues 142-515 of DELE1 (represent…

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcs2-cDELE1(142-515)-GFP-IRES-mCherry

    Plasmid

    #226102

    Purpose

    cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) stability reporter construct for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    DELE1 (DELE1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    only encodes for residues 142-515 of DELE1 corres…

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. gamma-tubulin (1-335)

    Plasmid

    #226519

    Purpose

    Expressing a fragment of gamma-tubulin containing gamma-tubulin GTPase domain

    Depositor

    Maria Alvarado-Kristensson

    Article

    Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

    Insert

    TUBG1 N-terminal amino acids 1-335 (TUBG1 Human)
    

    Use

    Pcdna3.1

    Expression

    Mammalian

    Mutation

    Deleted amino acids 334-451 and introduced the fo…

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-Regnase-3 D252N-T2A-copGFP

    Plasmid

    #222661

    Purpose

    Lentiviral vector to express mouse Regnase-3 D252N

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c (Zc3h12c Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    mutated aspartic acid 252 to asparagine to abroga…

    Promoter

    EF1α

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 D141N

    Plasmid

    #222650

    Purpose

    For in vitro transcription of mouse Zc3h12a D141N mutant tagged with FLAG-HA at N-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12a (Zc3h12a Mouse)
    

    Use

    In vitro transcription

    Tags

    FLAG and HA

    Mutation

    mutated aspartic acid 141 to asparagine to abroga…

    Promoter

    T7

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNK189

    Plasmid

    #219743

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Homo sapiens

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mutant of fungal hispidin-3-hydroxylase
    

    Use

    Synthetic Biology

    Mutation

    D37E, V181I, S323M, M385K

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNK4169

    Plasmid

    #219744

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastoris

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mutant of fungal hispidin-3-hydroxylase
    

    Use

    Synthetic Biology

    Mutation

    D37E, V181I, S323M, M385K

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFB1.HMBP.PrS.EZH2-mt2

    Plasmid

    #220240

    Purpose

    Expression of an EZH2 mutant in insect cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 2 (EZH2 Human)
    

    Tags

    6x His, MBP

    Expression

    Insect

    Mutation

    F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

    Promoter

    Polyhedrin

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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