We narrowed to 10,238 results for: ada

1. MSCV-HA-Nr4a1-Zf2

   Plasmid

   #160944

   Purpose

   Generation of retrovirus for the overexpression of Nr4a1 mutant (zinc finger 2 deletion)

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Nr4a1 (Nr4a1 Mouse)
   

   Use

   Retroviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Deletion of Zinc finger 2 (C306 to C330)

   Promoter

   MSCV-LTR

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-HA-Nr4a1-Zf1

   Plasmid

   #160943

   Purpose

   Generation of retrovirus for the overexpression of Nr4a1 mutant (zinc finger 1 deletion)

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Nr4a1 (Nr4a1 Mouse)
   

   Use

   Retroviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Deletion of Zinc finger 1 (C270 to C290)

   Promoter

   MSCV-LTR

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. DHC1-mIAA7 Neo

   Plasmid

   #140543

   Purpose

   DHC1 tagging with mIAA7

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   DHC1 (DYNC1H1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-IRES-Blast POLA1 ISCmut

   Plasmid

   #160808

   Purpose

   Express ISC mutant POLA1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLA1 ISCmut (POLA1 Human)
   

   Use

   Retroviral

   Mutation

   Codon optimized, C1354S, C1359S, C1377S, C1380S

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-eGFP-FRT-T0-RNF168 delta MIU1/MIU2

   Plasmid

   #133980

   Purpose

   inducible mammalian expression vector of eGFP tagged RNF168 where both motifs interacting with ubiquitin have been deleted

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   deletion of MIU1 and MIU2

   Promoter

   CMV

   Available Since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. gRNA-NONO-1

   Plasmid

   #127654

   Purpose

   Knock-out of human NONO (guide only)

   Depositor

   Nicolas Manel

   Article

   Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

   Insert

   NONO sgRNA (NONO Human)
   

   Expression

   Mammalian

   Available Since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI-TPI_WT-TNFalpha_3UTR-xrRNA-4H

   Plasmid

   #108374

   Purpose

   Expresses wild type TPI reporter; contains partial TNFalpha 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

   Depositor

   Niels Gehring

   Article

   Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

   Insert

   Wild type TPI reporter with partial TNFalpha control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, TNF Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI-TPI_WT-RAB7A_3UTR-xrRNA-4H

   Plasmid

   #108372

   Purpose

   Expresses wild type TPI reporter; contains partial RAB7A control 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

   Depositor

   Niels Gehring

   Article

   Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

   Insert

   Wild type TPI reporter with partial RAB7A control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, RAB7A Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-4T-3 SIRT1-NTerm Exon1-Exon3

   Plasmid

   #105681

   Purpose

   Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon3 (deltaExon2)

   Depositor

   Ullas Kolthur-Seetharam

   Article

   Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

   Insert

   SIRT1-NTerm: Exon1-Exon3 (Sirt1 Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Synonymous base changes not affecting the protein…

   Promoter

   tac_Promoter

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3

    Plasmid

    #105680

    Purpose

    Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon2-Exon3

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1-NTerm: Exon1-Exon2-Exon3 (Sirt1 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    tac_Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLuc-Optop38i-dsm

    Plasmid

    #89748

    Purpose

    Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    Optop38i dark-state mutant
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    C450A mutation of AsLov2Jalpha

    Promoter

    CMV

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV2_hSyn_Aurora_CA_Citrine

    Plasmid

    #98219

    Purpose

    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

    Depositor

    Simon Wiegert

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Aurora_C128A gene
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

    Promoter

    human synapsin

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDF-casBCDE(-6)

    Plasmid

    #89730

    Purpose

    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-6) truncated by 6 nucleotides at the leader-distal end.

    Depositor

    Konstantin Kuznedelov, Konstantin Severinov

    Article

    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    CRISPR array
    

    Expression

    Bacterial

    Mutation

    Derivative CRISPR array containing a shorter vers…

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDF-casBCDE(-12)

    Plasmid

    #89731

    Purpose

    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-12) truncated by 12 nucleotides at the leader-distal end.

    Depositor

    Konstantin Kuznedelov, Konstantin Severinov

    Article

    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    CRISPR array
    

    Expression

    Bacterial

    Mutation

    Derivative CRISPR array containing a shorter vers…

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDF-casBCDE(-18)

    Plasmid

    #89732

    Purpose

    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-18) truncated by 18 nucleotides at the leader-distal end.

    Depositor

    Konstantin Kuznedelov, Konstantin Severinov

    Article

    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    CRISPR array
    

    Expression

    Bacterial

    Mutation

    Derivative CRISPR array containing a shorter vers…

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDF-casBCDE(-3)

    Plasmid

    #89729

    Purpose

    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-3) truncated by 3 nucleotides at the leader-distal end.

    Depositor

    Konstantin Kuznedelov, Konstantin Severinov

    Article

    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    CRISPR array
    

    Expression

    Bacterial

    Mutation

    Derivative CRISPR array containing a shorter vers…

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLXSN p200 CUX1

    Plasmid

    #90470

    Purpose

    Retroviral vector expressing human p200 CUX1 with a Myc and HA tag at the N- and C-terminus, respectively

    Depositor

    Alain Nepveu

    Article

    Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11

    Insert

    CUX1 (CUX1 Human)
    

    Use

    Retroviral

    Tags

    Ha and myc

    Expression

    Mammalian

    Promoter

    Moloney murine leukemia virus long terminal repeat

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #190033

    Purpose

    vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized hfCas13X
    

    Expression

    Mammalian

    Mutation

    Y672A,Y676A

    Promoter

    CBh, SV40, hU6

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #190034

    Purpose

    vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized hfCas13d
    

    Expression

    Mammalian

    Mutation

    A134V,A140V,A141V,A143V

    Promoter

    CBh, SV40, hU6

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-PEmax-P2A-hMLH1dn

    Plasmid

    #174828

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-hMLH1dn
    

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript; deletion of residues 754-…

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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