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pAAV_hSyn-PdCO-mScarlet-WPRE

Plasmid

#198510

Purpose

Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has Service

AAV1 and AAV5

Insert

PdCO

Use

AAV

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

hSyn1

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTwist-CMV-HDGFL2_Cryptic_TwinstrepTEV_6xHis

Plasmid

#232344

Purpose

Expresses human HDGFL2 protein with TDP-43 regulated cryptic exon, along with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag

Depositor

Philip Wong

Article

Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10

Insert

HDGF Like 2 (HDGFL2 Human)

Tags

GGGS linker, 6xHis and Twin-strep, TEV protease s…

Expression

Mammalian

Mutation

TDP-43 regulated cryptic exon

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn-DIO-PdCO-mScarlet-WPRE

Plasmid

#198511

Purpose

Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has Service

AAV1 and AAV5

Insert

PdCO

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

hSyn1

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTPN11-N308D

Plasmid

#116644

Purpose

Lentiviral expression of PTPN11 N308D

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTPN11 (PTPN11 Human)

Use

Lentiviral

Mutation

N308D

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) EPB41L1

Plasmid

#107508

Purpose

Expressing EPB41L1, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

EPB41L1 (EPB41L1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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retro-gfpIkkb-puro vector

Plasmid

#58251

Purpose

Retroviral expression vector encoding bicistronic EGFP-IKKbeta and Puromycin cassette

Depositor

Georgios Stathopoulos

Article

Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

Insert

Ikkβ (Ikbkb Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-VGAT [L118/80R]

Plasmid

#177463

Purpose

Mammalian Expression Plasmid of anti-VGAT (Mouse). Derived from hybridoma L118/80.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VGAT (Mus musculus) recombinant mouse monoclonal antibody (Slc32a1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pEF2-EFGR-hMer

Plasmid

#225996

Purpose

Expresses a chimeric construct having N-terminal human EGFR and C-terminal human MerTK

Depositor

Raymond Birge, Sergei Kotenko

Article

Kimani et al Cell Commun Signal. 2016 Sep 6;14(1):19.

Insert

human EGFR N-Terminal human MerTK C-Terminal Fusion (EGFR, MERTK Human)

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Kv7.2/KCNQ2 K+ channel [N26A/23R]

Plasmid

#177524

Purpose

Mammalian Expression Plasmid of anti-Kv7.2/KCNQ2 K+ channel (Human). Derived from hybridoma N26A/23.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv7.2/KCNQ2 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody (KCNQ2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Kv1.4 K+ channel (extracellular) scFv [L71/5]

Plasmid

#190517

Purpose

Mammalian Expression of Kv1.4 K+ channel (extracellular) scFV. Derived from hybridoma L71/5.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv1.4 K+ channel (extracellular) (Rattus norvegicus) recombinant scFV (Kcna4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa(0.4)-PdCO-mScarlet-WPRE

Plasmid

#198508

Purpose

Expresses optimized PdCO in frame with mScarlet under control of minimal CamKIIa promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

AAV

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

CaMKIIα minimal promotor (0.4kb)

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Alpha1 [N95/35R]

Plasmid

#177574

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Alpha1 (Human). Derived from hybridoma N95/35.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Alpha1 (Homo sapiens) recombinant mouse monoclonal antibody (GABRA1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Cav3.2 Ca2+ channel [N55/10R]

Plasmid

#177562

Purpose

Mammalian Expression Plasmid of anti-Cav3.2 Ca2+ channel (Human). Derived from hybridoma N55/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Cav3.2 Ca2+ channel (Homo sapiens) recombinant mouse monoclonal antibody (CACNA1H Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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18065-M01-412

Plasmid

#225664

Purpose

Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue

Depositor

Dominic Esposito

Article

Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

Inserts

dsCopGFP
delCD19 (CD19 Human)

Use

Lentiviral

Tags

3xFLAG

Mutation

C-terminally truncated (aa 1-333 only)

Promoter

SORE6-mCMVp and WPRE-SV40p

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-CASK [K56A/50R]

Plasmid

#177443

Purpose

Mammalian Expression Plasmid of anti-CASK (Human). Derived from hybridoma K56A/50.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-CASK (Homo sapiens) recombinant mouse monoclonal antibody (CASK Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Histone H4-dimethyl-Arg3 [N309A/21R]

Plasmid

#206552

Purpose

Mammalian Expression Plasmid of anti-Histone H4-dimethyl-Arg3 (Human). Derived from hybridoma N309A/21.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Histone H4-dimethyl-Arg3 (Homo sapiens) recombinant Mouse monoclonal antibody (H4C14 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-GluN1 [N308/48R]

Plasmid

#177529

Purpose

Mammalian Expression Plasmid of anti-GluN1/NR1 glutamate receptor (Rat). Derived from hybridoma N308/48.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GluN1/NR1 glutamate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody (Grin1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Beta3 [N87/25R]

Plasmid

#177573

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Beta3 (Mouse). Derived from hybridoma N87/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Beta3 (Mus musculus) recombinant mouse monoclonal antibody (Gabrb3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-AMIGO-1 [L86/2]

Plasmid

#190294

Purpose

Mammalian Expression Plasmid of anti-AMIGO-1 (Mouse). Derived from hybridoma L86/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-AMIGO-1 (Mus musculus) recombinant Mouse monoclonal antibody (Amigo1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Slo1/BKAlpha maxi-K+ channel scFv [L6/60]

Plasmid

#199421

Purpose

Mammalian expression of Slo1/BKAlpha maxi-K+ channel scFv. Derived from hybridoma L6/60.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingSlo1/BKAlpha maxi-K+ channel (Rat) (Kcnma1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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