We narrowed to 19,051 results for: Tec;

1. SPHK1 gRNA (BRDN0001147454)

   Plasmid

   #76813

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK1 (SPHK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRDB_053

   Plasmid

   #216083

   Purpose

   for transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vector

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
   

   Use

   Destination

   Expression

   Insect

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-HLA-Flag-natT1R3

   Plasmid

   #113950

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R3 (TAS1R3 Human)
   

   Tags

   FLAG and Signal/leader sequence from HLA class I …

   Expression

   Mammalian

   Mutation

   residues 21-852

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. SPHK1 gRNA (BRDN0001147534)

   Plasmid

   #76814

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK1 (SPHK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPHK2 gRNA (BRDN0001146882)

   Plasmid

   #77096

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK2 (SPHK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. M87

   Plasmid

   #242633

   Purpose

   MYC expression

   Depositor

   Alain Nepveu

   Article

   Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

   Insert

   EGFP-MYC (MYC Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. SPHK1 gRNA (BRDN0001148403)

   Plasmid

   #76815

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK1 (SPHK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9-NG-YE1-BE4max

   Plasmid

   #198535

   Purpose

   Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-YE1-BE4max
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL3718 (pQCascade_Pse)

   Plasmid

   #200899

   Purpose

   Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   PseCas7, PseCas8, PseCas6, PseTniQ
   

   Tags

   NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDisplay-GACh2.0

    Plasmid

    #106073

    Purpose

    express the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

    Insert

    GPCR activation based ACh sensor (GACh) 2.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEvol-pAzFRS.1.t1

    Plasmid

    #73547

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAzFRS.1.t1
    pAzFRS.1.t1
    

    Expression

    Bacterial

    Available Since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pY108 (lenti-AsCpf1)

    Plasmid

    #84739

    Purpose

    Lenti virus delivery of AsCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huAsCpf1
    puromycin resistance gene
    

    Use

    Lentiviral

    Tags

    3xHA, NLS, and P2A

    Promoter

    EFS

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Pseudojanin (PJ)

    Plasmid

    #37999

    Depositor

    Robin Irvine

    Article

    Hammond et al Science. 2012 Jun 21.

    Inserts

    FKBP1A (FKBP1A Human)
    SAC1
    INPP5E (INPP5E Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pY109 (lenti-LbCpf1)

    Plasmid

    #84740

    Purpose

    Lenti virus delivery of LbCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    puromycin resistance gene
    

    Use

    Lentiviral

    Tags

    3xHA, NLS, and P2A

    Promoter

    EFS

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHS2056

    Plasmid

    #239837

    Purpose

    OMEGAOff + omegaRNA scaffold AAV expression

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    DNMT3A-DNMT3L-NovaIscB-KRAB::NovaIscB omegaRNA scaffold
    

    Use

    AAV

    Tags

    HA and SV40 NLS

    Mutation

    Insertion of REC domain from NbaCas9-1 + E137K/E4…

    Promoter

    EFS; human U6

    Available Since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAVS1-TLR targeting vector

    Plasmid

    #64215

    Purpose

    Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Insert

    traffic light reporter (TLR) (AAVS1 Human)
    

    Use

    Human targeting

    Mutation

    see publication for details

    Promoter

    CAG

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. A*11:01:01:01

    Plasmid

    #203276

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*11:01:01:01 (HLA-A Human)
    

    Expression

    Mammalian

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-Bcl2

    Plasmid

    #17999

    Depositor

    Clark Distelhorst

    Article

    Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

    Insert

    Bcl-2 (BCL2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSP6-RNAP

    Plasmid

    #48143

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    rna polymerase SP6
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    V314A (numbering based on NCBI Sequence ID: NP_85…

    Available Since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-GRAB_NE1m

    Plasmid

    #123308

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based NE sensor GRAB_NE1m
    

    Use

    AAV

    Mutation

    contains a glycine-to-threonine mutation at posit…

    Promoter

    hSyn

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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