Purpose
This AND-AND-NOT-gate plasmid expresses dCas9 from a constitutive promoter. Its sgRNAs X & Y are repressible by sgRNAs A & B, respectively. Its GFP gene is repressible by any of sgRNAs X, Y, and C.
Inserts
dCas9
GFP
sgRNA-X
sgRNA-Y
Available Since
Feb. 19, 2025
Availability
Academic Institutions and Nonprofits only