We narrowed to 96,015 results for: plasmid

1. pGBW-m4046574

   Plasmid

   #148976

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. CD44I-c001

   Plasmid

   #175448

   Purpose

   Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N172.

   Depositor

   Opher Gileadi

   Insert

   CD44 (CD44 Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pH3F

   Plasmid

   #85773

   Purpose

   N-ICE plasmid pH3F

   Depositor

   Scott Briggs

   Article

   Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

   Insert

   loxP-HphMX-loxP-3FLAG
   

   Use

   Cre/Lox

   Expression

   Yeast

   Available Since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Col7A1-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124287

   Purpose

   sgRNA against human Col7A1

   Depositor

   Katalin Josvay, Csaba Vizler

   Insert

   Collagen type VII alpha 1 chain (COL7A1 Human)
   

   Use

   CRISPR

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

5. pGBW-m4046286

   Plasmid

   #149147

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 surface glycoprotein (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   May 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pISlin41w(s) (lin-41 microRNA wt reporter)

   Plasmid

   #11906

   Depositor

   David Bartel

   Article

   Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

   Insert

   lin-41 miRNA target sequence (lin-41 Nematode)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   April 27, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJ291_WT_low

   Plasmid

   #128855

   Purpose

   Low copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

   Depositor

   Mark Pallen

   Article

   Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

   Insert

   Flagellin
   

   Expression

   Bacterial

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. JARID1BA

   Plasmid

   #74648

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   JARID1BA (KDM5B Human)
   

   Use

   Baculovirus

   Tags

   His-6-TEV

   Mutation

   Leu101-(Gly)4-Ala374 deletion mutant

   Promoter

   PH

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. p183_LTJ_sgRNACD90.2_A

   Plasmid

   #82671

   Purpose

   sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   sgRNA targeting CD90.2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMLX-donor

    Plasmid

    #112370

    Purpose

    CRISPR donor plasmid to tag human transcription factor MLX with GFP

    Depositor

    Kevin White

    Insert

    MLX homology arms flanking EGFP-IRES-Neo cassette (MLX Human)
    

    Use

    CRISPR

    Mutation

    hg38:17:42570798:G>A, hg38:17:42570950:T>G,…

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGBW-m4164344

    Plasmid

    #153554

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    accatg Kozak-START linker

    Expression

    Mammalian

    Mutation

    Gordon et al recoded

    Available Since

    June 16, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pJ211_rha_WT_med

    Plasmid

    #128852

    Purpose

    Medium copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Expression

    Bacterial

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pK3F-PYK1

    Plasmid

    #85771

    Purpose

    N-ICE plasmid pK3F-PYK1

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-KanMX-loxP-PYK1p-3FLAG
    

    Use

    Cre/Lox

    Expression

    Yeast

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Col7A1-CRISPR-gRNA#exon2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124286

    Purpose

    sgRNA against human Col7A1

    Depositor

    Katalin Josvay, Csaba Vizler

    Insert

    Collagen type VII alpha 1 chain (COL7A1 Human)
    

    Use

    CRISPR

    Available Since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

    Plasmid

    #99696

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV

    Depositor

    George Church

    Insert

    dCas9 and gRNA targeting Afp (Afp Synthetic)
    

    Use

    AAV

    Tags

    VPR mini

    Mutation

    dead Cas9

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4_HA-Halo-cmyc-GRM3 C127A

    Plasmid

    #140458

    Purpose

    Mammalian expression plasmid for N-terminal Halo- and c-myc-tagged mGluR3 C127A

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Tags

    Halo, Signal/leader sequence from influenza hemag…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, Threonine 174 to alanine

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898

    Plasmid

    #105635

    Purpose

    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid

    Depositor

    Thanos Halazonetis

    Inserts

    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    

    Expression

    Bacterial

    Mutation

    Mutations: F11S, G12A, V132K, C133V, A135K

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. CD44I-c011

    Plasmid

    #175449

    Purpose

    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N178. Used for crystallography.

    Depositor

    Opher Gileadi

    Insert

    CD44 (CD44 Human)
    

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-Clover-HIF-1alpha S31D

    Plasmid

    #163370

    Purpose

    Express HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser31Asp) plasmid

    Depositor

    Violaine Sée

    Insert

    Hypoxia Inducible Factor 1 alpha S31D (HIF1A Human)
    

    Tags

    HA- and Clover-tagged

    Expression

    Mammalian

    Mutation

    S31D

    Promoter

    CMV Promoter

    Available Since

    Jan. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-Clover-HIF-1alpha S31G

    Plasmid

    #163369

    Purpose

    Express HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser31Gly) plasmid

    Depositor

    Violaine Sée

    Insert

    Hypoxia Inducible Factor 1 alpha S31G (HIF1A Human)
    

    Tags

    HA- and Clover-tagged

    Expression

    Mammalian

    Mutation

    S31G

    Promoter

    CMV Promoter

    Available Since

    Jan. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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