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pACYC.GFP1Amb

Plasmid

#102792

Purpose

Expresses GFP with a premature stop codon

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Green Fluorescent Protein

Expression

Bacterial

Mutation

Y39Stop

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMT Shark-TagBFP

Plasmid

#126658

Purpose

Inducible expression of Shark-TagBFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Shark

Expression

Insect

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMT Stat92E-GFP

Plasmid

#126662

Purpose

Inducible expression of Stat92E-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Stat29E

Expression

Insect

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMT Lifeact-TagBFP

Plasmid

#126664

Purpose

Inducible expression of Lifeact-TagBFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Lifeact-TagBFP fusion

Expression

Insect

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAG426Gal-GFP

Plasmid

#38310

Depositor

Susan Lindquist

Article

McLellan et al ACS Chem Biol. 2012 Jun 22.

Insert

EGFP (eGFP )

Expression

Yeast

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pQE-WT-KIF-R5-IRH

Plasmid

#63629

Purpose

K1F-R5-IRH RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

KIF-R5-IRH RNAP

Mutation

L749I, Q754S, R756N, I761V, H772R, and Q786H

Promoter

T5/Lac

Available Since

June 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMT p85-GFP

Plasmid

#126660

Purpose

Inducible expression of p85-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

PI3K SH2 containing subunit

Expression

Insect

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pME-NLS-tdMCP

Plasmid

#86245

Purpose

Middle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Type

Empty backbone

Use

Unspecified

Tags

NLStdMCP

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCDW114

Plasmid

#70061

Purpose

artificial transcription template "wild-type"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' - repeat cassette - E. coli rpoB - lambda TR'

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
pCDW115

Plasmid

#70062

Purpose

artificial transcription template "pause ablation"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' A+2G T+6G - repeat cassette - E. coli rpoB - lambda TR'

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
LT1_72_TU6of6_X2homology

Plasmid

#194414

Purpose

TU6 backbone (for TU #6 for plasmid with 6 TUs) with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_77_TU1of2-6_HOhomology

Plasmid

#194416

Purpose

TU1 backbone (for TU #1 for plasmid with 2-6 TUs) with HO homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_78_TU2of2_HOhomology

Plasmid

#194417

Purpose

TU2 backbone (for TU #2 for plasmid with 2 TUs) with HO homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_79_TU2of3-6_HOhomology

Plasmid

#194418

Purpose

TU2 backbone (for TU #2 for plasmid with 3-6 TUs) with HO homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_80_TU3of3_HOhomology

Plasmid

#194419

Purpose

TU3 backbone (for TU #3 for plasmid with 3 TUs) with HO homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_81_TU3of4-6_HOhomology

Plasmid

#194420

Purpose

TU3 backbone (for TU #3 for plasmid with 4-6 TUs) with HO homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_82_TU4of4_HOhomology

Plasmid

#194421

Purpose

TU4 backbone (for TU #4 for plasmid with 4 TUs) with HO homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_83_TU4of5-6_HOhomology

Plasmid

#194422

Purpose

TU4 backbone (for TU #4 for plasmid with 5-6 TUs) with HO homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_84_TU5of5_HOhomology

Plasmid

#194423

Purpose

TU5 backbone (for TU #5 for plasmid with 5 TUs) with HO homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_85_TU5of6_HOhomology

Plasmid

#194424

Purpose

TU5 backbone (for TU #5 for plasmid with 6 TUs) with HO homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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