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pSUPER-retro-puro-shNup88-XbaI

Plasmid

#87328

Purpose

To express shRNA against human Nup88

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup88

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-CMV.Pro

Plasmid

#79486

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5), CMV promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CMV Promoter

Use

Multisite gateway entry clone

Promoter

CMV Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Meis3

Plasmid

#84312

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Meis3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Meis3 (Meis3 Mouse)

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Alx3

Plasmid

#84305

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Alx3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Alx3 (Alx3 Mouse)

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Dmbx1

Plasmid

#84308

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dmbx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Dmbx1 (Dmbx1 Mouse)

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Barx2

Plasmid

#84307

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Barx2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Barx2 (Barx2 Mouse)

Available Since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Alx1

Plasmid

#84304

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Alx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Alx1 (Alx1 Mouse)

Available Since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch5_155183064-gRNA-rev

Plasmid

#81215

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch5 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pHU6_gRNA_Ch10-for2

Plasmid

#81209

Purpose

for targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs from the cas9 site and 3' of gix site)

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-mCE

Plasmid

#82459

Purpose

Expression of myc epitope and murine mRNA Capping Enzyme in mammalian cells

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

mRNA capping enzyme (Rngtt Mouse)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Scmh1-3'UTR

Plasmid

#61785

Purpose

Luciferase reporter containing the 3'UTR of mouse Scmh1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Scmh1 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Crtap-3'UTR

Plasmid

#61772

Purpose

Luciferase reporter containing the 3'UTR of mouse Crtap

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Crtap 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pISH-Dlx4

Plasmid

#74343

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx4. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

distal-less homeobox 4 (Dlx4 Mouse)

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pNH (GB0064)

Plasmid

#68165

Purpose

Provides the S. Lycopersicum fruit promoter NH as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlNH promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPUbc (GB0145)

Plasmid

#68170

Purpose

Provides the S. Lycopersicum Ubiquitin carrier protein promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlUbc promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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p2x35S (GB0222)

Plasmid

#68173

Purpose

Provides the CaMV dual 35S Promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

2x35S promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only
pUAST attB BmOr2 (Y464A)

Plasmid

#73927

Purpose

UAS induced expression of Bombyx mori odorant receptor 2 (OR2) cDNA with 464 amino acid change of Tyrosine to Alanine

Depositor

Leslie Vosshall

Article

Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

Insert

BmOr2 (Y464A)

Expression

Insect

Mutation

changed 464 Tyrosine to Alanine

Promoter

hsp70

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-Vv4CL-PhCHS-MsCHI

Plasmid

#73400

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Vv4CL
PhCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-Vv4CL-CmCHS-CmCHI

Plasmid

#73403

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Vv4CL
CmCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-Pc4CL-CmCHS-CmCHI

Plasmid

#73399

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
CmCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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