We narrowed to 25,078 results for: mpl

1. pcDNA3 Flag WDR5

   Plasmid

   #15552

   Depositor

   Kai Ge

   Article

   Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

   Insert

   Wdr5 (Wdr5 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. 809 pcDNA3 GFP PTEN

   Plasmid

   #10759

   Depositor

   William Sellers

   Article

   Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMaCTag-P28

   Plasmid

   #120039

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHA

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   3xHA
   

   Use

   Pcr template

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDF0387 pcmv-hudiscas7-11-r978-gggs-r1294

   Plasmid

   #186984

   Purpose

   mammalian expression of cas7-11-r978-gggs-r1294

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   cas7-11-r978-gggs-r1294
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   r978-gggs-r1294 deletion

   Promoter

   Available since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya

   Plasmid

   #186980

   Purpose

   mammalian expression of Cluc-Gluc reporter

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   Cluc, Gluc
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-PAK2 binding domain

   Plasmid

   #83951

   Purpose

   FLIM acceptor for mEGFP Rac1

   Depositor

   Ryohei Yasuda

   Article

   Hedrick et al Nature. 2016 Sep 28;538(7623):104-108. d

   Insert

   Pak2 (PAK2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   aa60-113 only; S74A F84A

   Promoter

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJAT11

   Plasmid

   #204290

   Purpose

   For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-DsRed
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ie1

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

   Plasmid

   #55220

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   His6 and MBP, Asn10

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. 6xHis-MBP-huErCas12a

   Plasmid

   #174685

   Purpose

   Expresses 6xHis-MBP tagged ErCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huErCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV, NLS and NLS, 6xHis

   Expression

   Bacterial

   Mutation

   Q926R mutation is observed as indicated from the …

   Promoter

   T7

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUAP4

    Plasmid

    #136079

    Purpose

    Acceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. FLAG-MRGBP

    Plasmid

    #15325

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Cai et al J Biol Chem. 2003 Oct 31. 278(44):42733-

    Insert

    MRG-binding protein (MRGBP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pTP341 pHAGE2 CMV TagBFP-3xFLAG

    Plasmid

    #199391

    Purpose

    Lentiviral transfer plasmid for constitutive expression of a TagBFP-3xFLAG in mammalian cells

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    TagBFP-3xFLAG
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. His6_MBP_FXR2P_P474S_P493N

    Plasmid

    #198695

    Purpose

    Expression of human FXR2 protein in E. coli

    Depositor

    Simpson Joseph

    Article

    Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

    Insert

    FXR2P (FXR2 Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Changed proline 474 to serine and proline 493 asp…

    Promoter

    T7

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Col2.3deltaTK

    Plasmid

    #114273

    Purpose

    Conditional ablation of mature bone cells via ganciclovir exposure of cells expressing a truncated thymidine kinase.

    Depositor

    David Rowe

    Article

    Visnjic et al J Bone Miner Res. 2001 Dec;16(12):2222-3

    Insert

    Col2.3 promoter + deltaTK + BGH polyA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    2.3 kb rat type 1 collagen

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ESCRT-I

    Plasmid

    #17634

    Depositor

    James Hurley

    Article

    Kostelansky et al Cell. 2006 Apr 7. 125(1):113-26.

    Insert

    Vps23(1-385) – His/Vps28(1-242) – Vps37(1-213) – Mvb12(1-101) (VPS28, STP22, SRN2 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMBP-SpyCatcher

    Plasmid

    #79053

    Purpose

    Expresses maltose binding protein (MBP) SpyCatcher fusion

    Depositor

    James Bowie

    Article

    Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

    Insert

    MBP-SpyCatcher
    

    Use

    Tags

    6xHis and Maltose binding protein

    Expression

    Bacterial

    Mutation

    Derived from pDEST14-SpyCatcher (Plasmid #35044)…

    Promoter

    T7

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-MS2-HA-HsSmg5_T

    Plasmid

    #147552

    Purpose

    Mammalian Expression of HsSmg5

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsSmg5 (SMG5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastBac1 INI1

    Plasmid

    #1953

    Depositor

    Robert Kingston

    Article

    Phelan et al Mol Cell 1999 Feb;3(2):247-53.

    Insert

    INI1 (SMARCB1 Human)
    

    Use

    Tags

    flag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pMaCTag-Z05

    Plasmid

    #120048

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with eGFP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    eGFP
    

    Use

    Pcr template

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV5B-TGFbeta receptor I K232R

    Plasmid

    #11763

    Depositor

    Jeff Wrana

    Article

    Wieser et al EMBO J. 1995 May 15. 14(10):2199-208.

    Insert

    TGFbeta receptor I (TGFBR1 Human)
    

    Use

    Tags

    6xHIS

    Expression

    Mammalian

    Mutation

    K232R: kinase dead

    Promoter

    CMV

    Available since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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