Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 13,353 results for: BASE

Showing: 7941 - 7960 of 13353 results

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qTAG-C-sTagRFP-Blast

Plasmid

#207712

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Puro-sTagRFP

Plasmid

#207690

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-sTagRFP cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pHYX143

Plasmid

#202816

Purpose

Control plasmid expressing mScarlet-I fluorescent protein.

Depositor

Jean-Félix Dallery

Article

Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

Insert

mScarlet-I

Expression

Yeast

Promoter

ScPCK1

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pHYX173

Plasmid

#202817

Purpose

Plasmid expressing the TEV protease, codon-optimized for Saccharomyces cerevisiae.

Depositor

Jean-Félix Dallery

Article

Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

Insert

TEV protease

Expression

Yeast

Promoter

ScPCK1

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCW-LIC-N8C_Fab5a

Plasmid

#203693

Purpose

Expresses N8C_Fab5a

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab5a

Expression

Bacterial

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pCW-LIC-N8C_Fab2a

Plasmid

#203688

Purpose

Expresses N8C_Fab2a

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab2a

Expression

Bacterial

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pCW-LIC-N8C_Fab1b

Plasmid

#203691

Purpose

Expresses N8C_Fab1b

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab1b

Expression

Bacterial

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-145-146

Plasmid

#198600

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-145-146

Expression

Bacterial

Promoter

araBAD promoter

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-169-170

Plasmid

#198612

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-169-170

Expression

Bacterial

Promoter

araBAD promoter

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQC.Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)]-Fluc

Plasmid

#200124

Purpose

Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] P2A firefly luciferase

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pQC.Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc

Plasmid

#200125

Purpose

Retroviral expression of Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[CoVA] P2A firefly luciferase

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

Plasmid

#199254

Purpose

Expression construct encoding the high specificity SpCas9-KARA-D10A nickase

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

High specificity S. pyogenes Cas9-D10A-KARA nickase

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A K848A R1060A

Promoter

CAG promoter

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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AP76_pU.CAG.Cas9-D10A-K848A.rBGpA

Plasmid

#199253

Purpose

Expression construct encoding the high specificity SpCas9-KA-D10A nickase

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

High specificity S. pyogenes Cas9-D10A-KA nickase

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A K848A

Promoter

CAG promoter

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-Ca0.5

Plasmid

#198632

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Ca0.5

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-Ca0.2

Plasmid

#198631

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Ca0.2

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-Ca1

Plasmid

#198633

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Ca1

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-159-162

Plasmid

#198621

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-159-162

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-163-166

Plasmid

#198622

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-163-166

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-167-170

Plasmid

#198623

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-167-170

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-171-174

Plasmid

#198624

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-171-174

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 7941 - 7960 of 13353 results

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