We narrowed to 1,231 results for: POLI

1. pCMV6-AN-DDK_S580A POLI

   Plasmid

   #131238

   Purpose

   Mammalian expression of S580A DNA polymerase iota with an N-terminal FLAG-tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains a point mutation converting Pol iota ser…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6-AN-DDK_3KA POLI

   Plasmid

   #131239

   Purpose

   Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A and K446A

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains point mutations that convert lysine resi…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 RLUC POLIRES FLUC

   Plasmid

   #45642

   Purpose

   Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

   Depositor

   Nahum Sonenberg

   Article

   Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

   Insert

   RLUC - IRES - FLUC
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEvolvR-enCas9-PolI3M-TBD

   Plasmid

   #113077

   Purpose

   Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   David Schaffer, John Dueber

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI3M-TBD
   

   Expression

   Bacterial

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV6-AN-HA_aa 1-420 POLI

   Plasmid

   #201582

   Purpose

   Expresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   DNA polymerase iota (POLI Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Expresses amino acids 1-420 of DNA polymerase iota

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJRM919_WT aa 1-419 His POLI

   Plasmid

   #201445

   Purpose

   Low copy vector for expressing the catalytic domain (amino acids 1-419) of human DNA polymerase iota in E. coli with an N-terminal 6x His tag. C-terminal truncation mutant of pJRM919_WT His POLI

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   DNA polymerase iota (POLI Human)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Expresses amino acids 1-449 of DNA polymerase iot…

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV6-AN-DDK_RKK/AAA POLI

   Plasmid

   #131240

   Purpose

   Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530A

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains point mutations that convert arginine re…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV6-AN-DDK_3KA + S580A POLI

   Plasmid

   #131241

   Purpose

   Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580A

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains point mutations that convert Pol iota se…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV6-AN-DDK_1-590 POLI

   Plasmid

   #131233

   Purpose

   Mammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains a 24 bp insertion within the Pol iota co…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV6-AN-DDK_1-420 POLI

    Plasmid

    #131234

    Purpose

    Mammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a 24 bp insertion within the Pol iota co…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge