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pCellFree_G03 PC

Plasmid

#67132

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PC (PC Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pFRT_TO_eGFP_CELF1

Plasmid

#106100

Purpose

Inducible gene expression of EGFP - CELF1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

CELF1 (CELF1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCellFree_G01

Plasmid

#67135

Purpose

Gateway destination vector for cell-free expression with N-term 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G02

Plasmid

#67136

Purpose

Gateway destination vector for cell-free expression with C-term 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCellulose

Plasmid

#36300

Depositor

Jay Keasling

Article

Bokinsky et al Proc Natl Acad Sci U S A. 2011 Dec 13;10

Insert

pCellulose

Use

Synthetic Biology

Promoter

Two promoters from the E coli genome drive two di…

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pCellFree_G04

Plasmid

#67138

Purpose

Gateway destination vector for cell-free expression with C-term eGFP 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

EGFP and HIS

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKVC

Plasmid

#145339

Purpose

Cell-free/mammalian expression vector for Zika virus capsid protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Capsid

Tags

8xHis and eGFP

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

April 29, 2023

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2

Plasmid

#145370

Purpose

Gateway destination vector for cell-free and mamalian expression with N-term 8xHis-tag and mCherry tag

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Type

Empty backbone

Tags

His and mCherry

Expression

Bacterial

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G07

Plasmid

#67141

Purpose

Gateway destination vector for cell-free expression with N-term myc-tag mCherry tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His-MYC and mCherry

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FlagHA_CELF1

Plasmid

#106099

Purpose

Inducible gene expression of FlagHA - CELF1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

CELF1 (CELF1 Human)

Tags

Flag and HA

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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