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Showing: 31 - 40 of 3396 results

  4. pX458-Dual-Guide-Donor-ShuttleOnly

    Plasmid
    #171098
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Article
    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    XbaI site added upstream of U6 promotor. GtoC mu…
    Promoter
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    171098-plasmid-map-sequence-id-336112

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  5. pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide

    Plasmid
    #186993
    Purpose
    AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guide
    Depositor
    Omar Abudayyeh, Jonathan Gootenberg
    Article
    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00
    Insert
    cas7-11-s1006-gggs-d122, non-targeting crRNA guide
    Use
    AAV
    Tags
    Expression
    Mutation
    s1006-gggs-d1221 deletion
    Promoter
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    186993-plasmid-map-sequence-id-372325

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  6. pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide

    Plasmid
    #186991
    Purpose
    AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guide
    Depositor
    Omar Abudayyeh, Jonathan Gootenberg
    Article
    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00
    Insert
    cas7-11-r1045-gggs-r1122, non-targeting crRNA guide
    Use
    AAV
    Tags
    Expression
    Mutation
    r1045-gggs-r1122 deletion
    Promoter
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    186991-plasmid-map-sequence-id-370002

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  7. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118158
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118158-plasmid-map-sequence-id-240404

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  8. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid
    #118159
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118159-plasmid-map-sequence-id-240446

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  9. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid
    #118160
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118160-plasmid-map-sequence-id-240351

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  10. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid
    #118157
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118157-plasmid-map-sequence-id-240364

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Showing: 31 - 40 of 3396 results