We narrowed to 115 results for: Free Use GFP

1. pMBA334

   Plasmid

   #214746

   Purpose

   High copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23119-RiboJ-RBS(21992)-gfpmut3
   

   Expression

   Bacterial

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMBA333

   Plasmid

   #214745

   Purpose

   Medium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23119-RiboJ-RBS(21992)-gfpmut3
   

   Expression

   Bacterial

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMBA332

   Plasmid

   #214744

   Purpose

   Low copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23119-RiboJ-RBS(21992)-gfpmut3
   

   Expression

   Bacterial

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBeast-pAhpC-RBS- LacZ

   Plasmid

   #190060

   Purpose

   LacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

   Depositor

   Jerome Bonnet

   Article

   Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

   Insert

   lacZ
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBeast-pT7-soxA

   Plasmid

   #190057

   Purpose

   Express the bacterial monomeric sarcosine oxidase enzyme under the control of the strong constitutif phage promoter T7

   Depositor

   Jerome Bonnet

   Article

   Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

   Insert

   SoxA
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBeast-pT7-lox

   Plasmid

   #190059

   Purpose

   Express the bacterial lactate oxidase enzyme under the control of the strong constitutif phage promoter T7

   Depositor

   Jerome Bonnet

   Article

   Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

   Insert

   lox
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBeast-pT7-codA

   Plasmid

   #190058

   Purpose

   Express the bacterial choline oxidase enzyme under the control of the strong constitutif phage promoter T7

   Depositor

   Jerome Bonnet

   Article

   Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

   Insert

   codA
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBzCas13b-gRNA-1

   Plasmid

   #164858

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   BzCas13b-gRNA-1
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLsCas13a-gRNA-1

   Plasmid

   #164865

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   LsCas13a-gRNA-1
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBzCas13b-gRNA-2

    Plasmid

    #164859

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-2
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBzCas13b-gRNA-3

    Plasmid

    #164860

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-3
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLsCas13a-gRNA-3

    Plasmid

    #164867

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-3
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLsCas13a-gRNA-2

    Plasmid

    #164866

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-2
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7RNAPNev-NB1

    Plasmid

    #216336

    Purpose

    Arabinose-inducible PBad promoter driving expression of the evolved N-terminal portion (residues 1-179) of T7RNAP translationally fused to anti-eGFP nanobody NB1

    Depositor

    Mark Styczynski

    Article

    McSweeney et al Sci Adv. 2025 Feb 21;11(8):eado6280. doi

    Insert

    T7RNAPN-NB1 fusion
    

    Expression

    Bacterial

    Available Since

    July 3, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)

    Plasmid

    #70191

    Purpose

    Derived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    ClpX (clpX )
    

    Available Since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21-pelB-LaG94-5

    Plasmid

    #228965

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-5
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21-pelB-LaG94-15

    Plasmid

    #228971

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-15
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21-pelB-LaG94-9

    Plasmid

    #228968

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-9
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21-pelB-LaG94-10

    Plasmid

    #228969

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-10
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21-pelB-LaG94-12

    Plasmid

    #228970

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-12, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-12
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge