We narrowed to 4,951 results for: LARGE

1. pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

   Plasmid

   #171019

   Purpose

   Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   LargeBit-SNAPtag- Beta-actin
   

   Use

   Lentiviral and Luciferase

   Tags

   Beta actin and LargeBiT

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

   Plasmid

   #171019

   Purpose

   Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   LargeBit-SNAPtag- Beta-actin
   

   Use

   Lentiviral and Luciferase

   Tags

   Beta actin and LargeBiT

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SV40 1: pBSSVD2005

   Plasmid

   #21826

   Depositor

   David Ron

   Insert

   SV40 Large T (SV40gp6 simian virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   likely expresses aa109-708 of the large T antigen

   Promoter

   Available since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SV40 1: pBSSVD2005

   Plasmid

   #21826

   Depositor

   David Ron

   Insert

   SV40 Large T (SV40gp6 simian virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   likely expresses aa109-708 of the large T antigen

   Promoter

   Available since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBABEneo mt T Ag

   Plasmid

   #133713

   Purpose

   Large T antigen mutant form in pBABEneo vector for mammalian expression.

   Depositor

   James A. DeCaprio

   Insert

   SV40gp6 (SV40gp6 SV40)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   aa 1-354 of T antigen (including aa 1-130); no p5…

   Promoter

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBABEneo mt T Ag

   Plasmid

   #133713

   Purpose

   Large T antigen mutant form in pBABEneo vector for mammalian expression.

   Depositor

   James A. DeCaprio

   Insert

   SV40gp6 (SV40gp6 SV40)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   aa 1-354 of T antigen (including aa 1-130); no p5…

   Promoter

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOpen-BsupolLF

   Plasmid

   #165563

   Purpose

   Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   Bsu DNA Polymerase I, Large Fragment
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pOpen-BsupolLF

   Plasmid

   #165563

   Purpose

   Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   Bsu DNA Polymerase I, Large Fragment
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pSG5 E107K T Ag

   Plasmid

   #133710

   Purpose

   Large T antigen mutant form in pSG5 vector for mammalian expression.

   Depositor

   James A. DeCaprio

   Article

   Zalvide et al Mol Cell Biol. 1995 Oct . 15(10):5800-10

   Insert

   SV40gp6 (SV40gp6 SV40)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E107K

   Promoter

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSG5 E107K T Ag

    Plasmid

    #133710

    Purpose

    Large T antigen mutant form in pSG5 vector for mammalian expression.

    Depositor

    James A. DeCaprio

    Article

    Zalvide et al Mol Cell Biol. 1995 Oct . 15(10):5800-10

    Insert

    SV40gp6 (SV40gp6 SV40)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E107K

    Promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET15b_Bst-LF

    Plasmid

    #153313

    Purpose

    Bacterial expression of the Bst Large Fragment

    Depositor

    Philippa Marrack, Drew Endy

    Insert

    Bst Large Fragment
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pET15b_Bst-LF

    Plasmid

    #153313

    Purpose

    Bacterial expression of the Bst Large Fragment

    Depositor

    Drew Endy, Philippa Marrack

    Insert

    Bst Large Fragment
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pCMV5M/rDlg1

    Plasmid

    #28259

    Depositor

    Jeffrey Frost

    Article

    Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

    Insert

    Dlg1 I3
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pCMV5M/rDlg1

    Plasmid

    #28259

    Depositor

    Jeffrey Frost

    Article

    Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

    Insert

    Dlg1 I3
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only

15. EcMscL

    Plasmid

    #92418

    Purpose

    E. coli Mechanosensitive Channel of Large Conductance

    Depositor

    Douglas Rees

    Insert

    MscL (mscL E. coli)
    

    Use

    Tags

    6x Histidine Tag and Thrombin Cleavage Site betwe…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EcMscL

    Plasmid

    #92418

    Purpose

    E. coli Mechanosensitive Channel of Large Conductance

    Depositor

    Douglas Rees

    Insert

    MscL (mscL E. coli)
    

    Use

    Tags

    6x Histidine Tag and Thrombin Cleavage Site betwe…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK539

    Plasmid

    #72515

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)

    Depositor

    T. J. Kappock

    Insert

    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK549

    Plasmid

    #72520

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)

    Depositor

    T. J. Kappock

    Insert

    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK539

    Plasmid

    #72515

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)

    Depositor

    T. J. Kappock

    Insert

    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK549

    Plasmid

    #72520

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)

    Depositor

    T. J. Kappock

    Insert

    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge