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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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In Category

We narrowed to 492 results for: LARGE

Showing: 1 - 20 of 492 results

  1. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...that can cause large genomic deletions. Compared to deletions generated by Cas9, large deletions created...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR... While abundant in nature, Class 1 systems are largely underutilized compared to their Class 2 counterparts...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources. Find...

  2. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...CRISPR-associated transposases (CASTs) combine the large-scale capacity of transposases (Tns) with the precise...CRISPR-Cas systems, allowing for the integration of large fragments of DNA at specific locations. The Cas ...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources. Find...

  3. Open Enzyme Collection

    Type
    Collection
    ...Polymerase I, Large (Klenow) Fragment 165522 pOpen-K12polLF (exo-) DNA Polymerase I, Large (Klenow) Fragment...Polymerase 165553 pOpen-BstpolLF Bst DNA Polymerase, Large Fragment 165562 pOpen-Pwopol Pwo DNA Polymerase ...Polymerase 165563 pOpen-BsupolLF Bsu DNA Polymerase I, Large Fragment 165570 pOpen-P45 P45 165572 pOpen-GBDpol ...

  4. CRISPR Plasmids and Resources

    Type
    Collection
    ...Cascade-Cas3 generates large genomic deletions. Insert CRISPR transposases insert large fragments of DNA. ...

  5. Adenovirus Plasmids

    Type
    Collection
    ...dividing and non-dividing cells, and relative ease of large-scale production. In order to produce recombinant... vector for use in AdEasy™ System, for use with large inserts Vogelstein 1000000176 AdenoBuilder Toolkit...

  6. CRISPR Guide

    Type
    Collection
    ... Browse Plasmids: Prime Edit Large Scale Edits CRISPR can generate large-scale genetic changes, including...CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening Cas9 Fusion Tools Cas9...knockout or gene edit; or using Cas9 to generate large genomic deletions or inversions with two gRNA sites...plasmid or linear dsDNA templates work better for large edits or insertions. When designing the donor template...Figure 4: Overview of HDR. In most applications, a large portion of the Cas9-induced DSBs will still be repaired...Transposon systems like piggyBac or Tn7 allow for large scale edits by integrating segments of DNA flanked..., developed by the Feng Zhang lab ) combine the large-scale capacity of transposases with the precise ...

  7. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ... and rapid advances in technologies that permit large-scale creation and synthesis (“writing”) of longer...-based sequencing and confirmation of Addgene’s large and growing collection of curated plasmids from ...technique in our partnership with Addgene to sequence large numbers of plasmids. plexWell™ technology overview...sequential “pooled library prep”, in which a relatively large number of samples are subjected to a DNA-barcoding...normalization problem often arises from the fact that large numbers of individual input DNA samples (e.g. a ...measured in time and consumables, can be daunting for large numbers of samples. As mentioned above, one of the... is that normalized libraries are produced from large numbers of samples with a wide range of input concentrations...

  8. Plan Your Experiment

    Type
    Collection
    ...edits, including point mutations, base changes, and large insertions or deletions. For more information on...efficiencies. The template sequences can also have a large impact on editing efficiency. So while testing multiple...of AAV vectors is that the packaging limit (how large of a DNA sequence you can actually package into ...edit. Even if you provided a homology template, a large percentage of DNA breaks will be repaired by NHEJ... Can't distinguish specific edits PCR Knockouts Large insertions/deletions Some prime and base edits Can...inefficient methods and often require screening a larger pool of cells to find one with your desired edit...

  9. AAV Packaged on Request

    Type
    Collection
    ...10 × 100 µL aliquots 1.0 mL Large 25 × 100 µL aliquots 2.5 mL Extra Large 50 × 100 µL aliquots 5.0 mL ...

  10. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources. Find...

  11. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources. Find...

  12. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...together without forming large oligomeric complexes. Antibodies are also too large to be expressed in bacteria...strength. This signal strength is due in large part to their larger size (~10-15 nm), which allows for the...antibody structures. Functional comparison Strong but Large: Advantages of Antibodies One of the main reasons...being big is a double-edged sword. An antibody’s large size creates more distance between the target antigen...ability to penetrate tissue. Because antibodies are large and bivalent, primary and secondary antibodies cannot... to 3 targets (Pleiner et al figure 4D). Unlike large secondary antibodies, small nanobodies do not oligomerize... Poor tissue penetration Less resolution due to larger label displacement Require separate incubation ...

  13. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources. Find...

  14. Zebrafish Plasmid Collection

    Type
    Collection
    ...of the embryo and larvae, it is easy to culture large populations that are amenable to genetics, high-...reporters, biosensors, and Cre expression plasmids. Large collections or kits are described below . ID Plasmid...

  15. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources. Find...

  17. CRISPR Plasmids - Plants

    Type
    Collection
    ...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources. Find...

  18. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources. Find...

  20. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources. Find...
Showing: 1 - 20 of 492 results