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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 469 results for: LARGE

Showing: 1 - 20 of 469 results

  1. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...that can cause large genomic deletions. Compared to deletions generated by Cas9, large deletions created...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR... While abundant in nature, Class 1 systems are largely underutilized compared to their Class 2 counterparts...

  2. Open Enzyme Collection

    Type
    Collection
    ...Polymerase I, Large (Klenow) Fragment 165522 pOpen-K12polLF (exo-) DNA Polymerase I, Large (Klenow) Fragment...Polymerase 165553 pOpen-BstpolLF Bst DNA Polymerase, Large Fragment 165562 pOpen-Pwopol Pwo DNA Polymerase ...Polymerase 165563 pOpen-BsupolLF Bsu DNA Polymerase I, Large Fragment 165570 pOpen-P45 P45 165572 pOpen-GBDpol ...

  3. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...CRISPR-associated transposases (CASTs) combine the large-scale capacity of transposases (Tns) with the precise...CRISPR-Cas systems, allowing for the integration of large fragments of DNA at specific locations. The Cas ...

  4. CRISPR Plasmids and Resources

    Type
    Collection
    ...Cascade-Cas3 generates large genomic deletions. Insert NEW CRISPR transposases insert large fragments of DNA...

  5. Adenovirus Plasmids

    Type
    Collection
    ...Advantages of adenoviruses in research include their large packaging capacity (>8 kb), high titers, and high... For recombining shuttle plasmids; for use with large inserts Vogelstein 179201 pAd5-B6 ∆E3-GFP Adenoviral...

  6. CRISPR Guide

    Type
    Collection
    ... Browse Plasmids: Prime Edit Large Scale Edits CRISPR can generate large-scale genetic changes, including...CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening Cas9 Fusion Tools Cas9...knockout or gene edit; or using Cas9 to generate large genomic deletions or inversions with two gRNA sites...plasmid or linear dsDNA templates work better for large edits or insertions. When designing the donor template...increase HDR efficiency. In most applications, a large portion of the Cas9-induced DSBs will still be repaired...Transposon systems like piggyBac or Tn7 allow for large scale edits by integrating segments of DNA flanked..., developed by the Feng Zhang lab ) combine the large-scale capacity of transposases with the precise ...

  7. Plan Your Experiment

    Type
    Collection
    ...edits, including point mutations, base changes, and large insertions or deletions. For more information on...efficiencies. The template sequences can also have a large impact on editing efficiency. So while testing multiple...of AAV vectors is that the packaging limit (how large of a DNA sequence you can actually package into ...edit. Even if you provided a homology template, a large percentage of DNA breaks will be repaired by NHEJ... Can't distinguish specific edits PCR Knockouts Large insertions/deletions Some prime and base edits Can...inefficient methods and often require screening a larger pool of cells to find one with your desired edit...

  8. AAV Packaged on Request

    Type
    Collection
    ...10 × 100 µL aliquots 1.0 mL Large 25 × 100 µL aliquots 2.5 mL Extra Large 50 × 100 µL aliquots 5.0 mL ...

  9. Zebrafish Plasmid Collection

    Type
    Collection
    ...of the embryo and larvae, it is easy to culture large populations that are amenable to genetics, high-...reporters, biosensors, and Cre expression plasmids. Large collections or kits are described below . ID Plasmid...

  12. Retrovirus Plasmids

    Type
    Collection
    ...LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression of Large T antigen for creation of immortalized...

  13. Antibody Plasmid Collection

    Type
    Collection
    ...Expressing Antibodies Antibody Plasmids Antibodies are large proteins made by the immune system that bind to ...

  14. Luciferase Plasmid Collection

    Type
    Collection
    ...but are less-than-ideal protein tags due to their large size (61 kDa and 36 kDa, respectively). And they...new window) : The NanoLuc® enzyme is split into Large (LgBiT; 18 kDa) and Small (SmBiT; 11 amino acids...

  15. Worm Expression Resources

    Type
    Collection
    ...fluorescent proteins, and Cre expression plasmids. Large collections or kits are described below and not ...

  16. Promega Plasmid Collection

    Type
    Collection
    ...structural complementation reporter system composed of a Large BiT (LgBiT; 18 kDa) subunit and a small complementary...

  17. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Browse CRISPR pooled libraries for large-scale functional screens. Available for knockout, activation...representation. CRISPR screening experiments require large quantities of cells and therefore, may not be suitable...

  18. TALEN Guide

    Type
    Collection
    ...sequence ( Figure 1A ). Scientists have identified a large number of zinc fingers that recognize various nucleotide...

  19. p53 Pathway

    Type
    Collection
    ...only one functional copy of the p53 gene causes a large reduction in tumor suppression activity, leading...

  20. Viral Production

    Type
    Collection
    ...properties of the final product, particularly in large animal studies. Endotoxin contamination is minimized...
Showing: 1 - 20 of 469 results